Influences on Soil Organic Carbon Levels in Southwest Washington Pasturelands

Item

Title
Influences on Soil Organic Carbon Levels in Southwest Washington Pasturelands
Creator
Wagner, Christina
Identifier
Thesis_MES_2023_WagnerC
extracted text
INFLUENCES ON
SOIL ORGANIC CARBON IN
SOUTHWEST WASHINGTON PASTURELANDS

by
Christina M. Wagner

A Thesis
Submitted in partial fulfillment
Of the requirements for the degree
Master of Environmental Studies
The Evergreen State College
June 2023

©2023 by Christina M. Wagner. All rights reserved.

This Thesis for the Master of Environmental Studies Degree
by
Christina M. Wagner

has been approved for
The Evergreen State College
by

_______________________________
Sarah T. Hamman, Ph.D.
Member of Faculty

June 9, 2023
___________________________
Date

ABSTRACT
Influences on Soil Organic Carbon in Southwest Washington Pasturelands
Christina M. Wagner

Interest in soil organic carbon sequestration is gaining traction worldwide, although the dynamic
nature of soil and the influences of climate and human interaction make accurate predictions
difficult. In the Pacific Northwest, the primary avenue for carbon sequestration has been through
the abundant forests. However, climate change and anthropogenic pressures may impact forest
carbon sinks in unpredictable ways. Soil carbon sequestration, on the other hand, offers what
may be an effective, less vulnerable alternative to forest carbon sinks. Assessment of soil organic
carbon is largely unexplored in the northwest. This study analyzed the effects of habitat types
and management on soil organic carbon levels in Southwest Washington. Unexpectedly, soil
organic carbon levels were highest in the Puget Lowlands Prairie soils rather than forest soil
types, a very surprising finding given the high sand content and shallow, rocky nature of Puget
Lowlands Prairie soils. Analysis of management practices, such as weed management, irrigation,
fertilization, application of soil amendments, pasture renovation, and tilling, were not conclusive,
although some trends were suggested. Comparison of pasture history, current use, animal
species, relative animal numbers, and grazing styles indicated management techniques that
support soil organic carbon accumulation. Southwest Washington soil is as diverse as its
agricultural operations, which complicated the analysis in this study. Nonetheless, the data
indicated that soil organic carbon sequestration is a viable climate mitigation tool.

Table of Contents

TABLE OF CONTENTS

IV

LIST OF FIGURES

VI

LIST OF TABLES

VII

ACKNOWLEDGEMENTS

VIII

CHAPTER 1. INTRODUCTION

1

CHAPTER 2. LITERATURE REVIEW

6

2.1 Climate change

6

2.2 Introduction to Soils
2.2.a Soil properties
2.2.a.1 Soil physical properties
2.2.a.2 Soil biological properties
2.2.a.3 Soil chemical properties
2.2.b Soil as an ecosystem
2.2.b.1 Ecosystem services
2.2.b.2 Role in climate change mitigation
2.2.b.2.i Carbon sequestration
2.2.b.2.ii Flood and drought resistance/resilience
2.2.c Soil analysis
2.2.c.1 Physical
2.2.c.2 Biological
2.2.c.3 Carbon sequestration stabilization
2.2.d Soil Texture

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11
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12
14
15
15
16
17
18
19
22
22
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2.3 Biomes in Southwest Washington
2.3.a Native Forest
2.3.b Native Prairies

25
25
26

2.4 Pasturelands
2.4.a Role in carbon cycle
2.4.b Management practices

28
29
31

2.5 Conclusion

35

CHAPTER 3. METHODS

37

CHAPTER 4. RESULTS

42
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4.1 Habitat type outcomes

43

4.2 Bulk density outcomes

47

4.3 Soil physical and chemical outcomes

52

4.4 Land management outcomes

55

4.5 Land use outcomes

62

4.6 Confounding factors

69

CHAPTER 5. DISCUSSION

70

5.1 Soil organic carbon

70

5.2 Land management

73

CHAPTER 6. CONCLUSION

76

REFERENCES

77

APPENDIX 1 MANAGEMENT SURVEY QUESTIONS

95

APPENDIX 2 MANAGEMENT SURVEY RESPONSES

99

APPENDIX 3 SOIL TEST RESULTS

118

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List of Figures
FIGURE 1. Bulk density corer and step probe with 12" (30.08 cm) soil core

39

FIGURE 2. Bulk density samples in the lab

40

FIGURE 3. Google map of survey sites

43

FIGURE 4. ESD effect on soil organic carbon

47

FIGURE 5. Linear regression of soil organic carbon with bulk density

48

FIGURE 6. ESD effect on bulk density

49

FIGURE 7. Bulk density core example

50

FIGURE 8. Weed management effect on bulk density

51

FIGURE 9. Grazing style effect on bulk density

52

FIGURE 10. Linear regression of soil organic carbon with soil silt percentage

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FIGURE 11. Linear regression of soil organic carbon with soil clay percentage

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FIGURE 12. Linear regression of soil organic carbon with cation exchange capacity

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FIGURE 13. Prior history effect on soil organic carbon

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FIGURE 14. Linear regression of soil organic carbon with start of current practices

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FIGURE 15. Weed management effect on soil organic carbon

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FIGURE 16. Land management effect on soil organic carbon

61

FIGURE 17. Current primary use effect on soil organic carbon

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FIGURE 18. Grazing style effect on soil organic carbon

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FIGURE 19. Linear regression of soil organic carbon with animal unit equivalent

66

FIGURE 20. Animal species effect on soil organic carbon

67

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List of Tables
TABLE 1. Soil textures of Southwest Washington Ecological Site Descriptions

25

TABLE 2. Site characteristics

44

TABLE 3. Site use

58

TABLE 4. Land management practices

68

vii

Acknowledgements
Many thanks are due to many people, including Evergreen faculty and staff: Kevin Francis,
Averi Azar, John Withey, John Kirkpatrick, Kathleen Saul, Shawn Hazboun, Ralph Murphy,
Steve Scheuerell. Thank you to the fellow members of Team Dirt: Derek Thedell, Claire
Kerwin, and Corey Franklin. Thank you to Chuck Francis, Sierra Smith, Stephen Bramwell,
Dani Gelardi, Marty Chaney, Erik Dahlke, Marcie Cleaver, Jake Yancey, Gina Smith, and
Adam Peterson for answering questions, offering insight, delivering equipment on a Saturday
morning, and helping focus this project. Teagan Wagner, Lily Wagner, and Tony Leung
were my home team and helped save my arthritic knees. A special thank you to Sarah
Hamman, my reader and all-around inspiration for me (and everyone else). Most of all, thank
you from the bottom of my heart to all 25 land managers for your time, for letting me sample
dirt on your property, pet your dogs, meet your horses, sheep, and cows, and for being
wonderful stewards of the land. I am grateful.

viii

Chapter 1. Introduction
Escalating climate change impacts—floods, droughts, extreme temperatures,
unpredictable precipitation patterns—drive scientists and leaders to find successful, costeffective, readily available, lasting mitigation solutions without harmful consequences (Lal,
2004; IPCC, 2021). Of the many approaches to alleviate climate change, the potential for soil
carbon sequestration is high, requires little new technology or equipment, is extremely low cost,
and may offset anthropogenic carbon emissions for decades if not longer (Lal, 2007). The
estimated 2344 Gt of organic carbon held in soil is the largest land-based carbon pool (Stockman
et al., 2012; He et al., 2016). Even at the lower end of the anticipated attainable soil carbon
sequestration capacity, the global soil organic carbon (SOC) sequestration rate is estimated to be
0.5Pg of carbon per year (Lorenz, 2018). In contrast, net forest ecosystems may sequester
1.7±0.5Pg of carbon per year (Lal, 2007). The difference between these two carbon pools is in
where the carbon is stored—below ground versus above ground—and how they each respond to
climate change impacts over time. This paper explores the nuances of soil carbon sequestration
and why it is a valuable, secure, long-term climate mitigation strategy.
However, accurate assessment of soil carbon stocks across landscapes is incredibly
difficult because of the dynamic nature of organic matter, the heterogeneity of inherent soil
properties, the variability of management practices, fluctuating climatic conditions, and the
temporal and spatial variations in carbon fractions. Soil carbon is an inherently responsive
component in soil, influenced by several factors, including the interplay of soil physical,
chemical, and biological properties (Amorim et al., 2020; Fu et al., 2021; Hudson, 1994; Naylor
et al., 2020; Sakin, 2012; Taboada et al., 2011).

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In the Pacific Northwest, extensive regional forests are traditionally viewed as the
primary carbon sink (Case et al., 2021). However, because of the difference in how and where
carbon is stored, grasslands have the potential to sequester larger amounts of carbon for a longer
time frame than forests (Bai and Cotrufo, 2022; Dass et al., 2018; Lorenz, 2018; Fu et al., 2021).
Grasslands, which include native prairies and pasturelands, contain roughly one third of the
terrestrial carbon worldwide in their soil (Bai and Cotrufo, 2022; Kim et al., 2023). In the United
States, grasslands are nearly a third of the land surface area and almost a quarter—51 million
hectares—of the privately held grazing lands (Schnabel et al., 2000; Havstad et al., 2009).
Consequently, small increases in soil carbon stocks in pasturelands may have significant impact
on climate mitigation goals, ranging from 0.02 to 1Mg of carbon per hectare per year.
Variations in sequestration rates are tied to climate, land use, and management practices
such as irrigation, fertilization, amendments, seeding, and grazing strategies (Lal, 2004, 2006,
2008; Taboada et al., 2011; Mudge et al., 2016; Abdalla et al., 2018; Khalil et al., 2019; Paustian
et al., 2019; Naidu et al., 2022; Kim et al., 2023). Active management of soil to enhance soil
carbon sequestration has proponents (Aguilera et al., 2016; Lal 2004, 2007, 2013, 2015, 2020)
and skeptics with reservations about both the universal capacity of soil to successfully sequester
significant amounts of carbon and the extent to which carbon sequestration will mitigate climate
change effects (He et al., 2016; Six et al., 2002; Yin et al., 2022). To advance understanding in
this area, this study asks, “What effects do habitat types and management practices have on
Soil Organic Carbon (SOC) in Southwest Washington pasturelands?”
Most soil carbon sequestration studies focus on long-term experiments in cropping
systems, where similar crops can be grown at sufficient scale over time to draw adequate
research data. Far fewer studies examine pasturelands, and those that do have been focused

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primarily on New Zealand, Australia, China, South America, Europe, Latin America, and the
Midwest and Southeastern US, where climate and edaphic conditions are dissimilar to the Pacific
Northwest (Abdalla et al., 2018). A large proportion of agricultural land—6% in Grays Harbor
County, 14% in Mason County, 25% in Thurston County, and 30% in Lewis County—in
Southwest Washington (SW WA) is dedicated to pastureland, making the study of soil organic
carbon levels in pasturelands salient over a large area (USDA NASS, 2017). I am not aware of
any studies that examine pasture management practices in SW WA. The region, which has a
unique combination of climate and soil features, may offer exceptional soil carbon sequestration
potential using pasture management practices to enhance natural carbon sequestration processes.
Assessing carbon sequestration rates over a multi-year timeframe is beyond the scope of
this study. Instead, in situ soil samples from each site paired with a management survey
capturing land use, land management practices, and grazing over a 20-year period sought to
determine the influence of those management practices on SOC levels. The sites included 23
pastures in 4 counties in SW WA as well as 3 restored native prairies within 2 counties. Midwest
Labs tested soil samples for soil organic matter percentage, total carbon percentage, available
phosphorus, extractable potassium, magnesium, calcium, hydrogen, pH, buffering capacity,
cation exchange capacity, and percent base saturation of cation elements. Bulk density (BD)
cores (a measure of soil mass per unit volume which has implications for soil porosity, water
holding capacity, and biological populations) were also extracted from each site. Laboratory
analysis of bulk density was conducted within the Evergreen Science Support Center.
Analysis of SOC levels in SW WA pasturelands showed consistently high levels of SOC
in one habitat type—Puget Lowland Prairie ecological sites—in comparison with Puget
Lowlands Forest, Moist Forest, Wet Forest, and Riparian Forest ecological sites. Soil organic

3

carbon levels’ negative association with soil textural components such as clay and silt
percentages are noteworthy. Grazing styles showed some influence on SOC levels, with higher
SOC levels for rotational styles and lower with continuous styles. Animal species grazing on the
pasturelands influenced SOC, not to a statistically significant level. The pre-2003 historical use
of the pastureland played a role in SOC for some sites. As expected, BD correlated inversely
with SOC levels, confirming previous findings that lower BD supports SOC accrual (Sakin,
2012). These findings indicate that despite concerns about SOC sequestration limitations due to
inherent soil properties, management of pasturelands can have a positive effect on SOC levels.
As scientists and political leaders seek solutions to mitigate anthropogenic climate
changes, the knowledge accrued in this study about SOC levels in Southwest Washington
grasslands will allow policy makers to devise incentive programs to reward agricultural
producers who are contributing to climate solutions with SOC sequestration in their pasturelands.
On 27 September 2022 the USDA announced its intention to support the development of a soil
carbon monitoring network with an investment of $8 million dollars to “train partners on soil
sampling and processing methods, conduct outreach to producers to use soil carbon monitoring
practices, coordinate with NRCS national and state centers for technical support, identify and
recruit specialists to help producers with soil carbon monitoring, and reach diverse producers to
participate in soil carbon monitoring and other NRCS conservation practices” (USDA NRCS,
2023). This is a growing field in which my work establishes baseline information and research
protocols, as well as provides a map for future studies by MES students and others.
Following the Chapter 1 Introduction, a thorough Literature Review in Chapter 2 offers a
brief context for the project, an overview of soils, and a comparison of the major biomes in
Southwest Washington. A deeper look at the role of pasturelands in the carbon cycle and

4

pastureland management practices concludes Chapter 2. Chapter 3, Methods, details the
development of the management survey, including recruitment, data collection, and data
assimilation. Site descriptions, field soil sampling, soil data collection, laboratory tests, and
statistical analysis are also included in the Methods chapter. Results (Chapter 4) and Discussion
(Chapter 5) examine in detail the data and analyses. The conclusion (Chapter 6) expresses the
primary implications of the findings and suggestions for further study.

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Chapter 2. Literature Review
2.1 Climate change
Globally, climate change, the long-term alteration of temperature and precipitation
patterns, has become an urgent priority. Addressing climate change impacts was named a top
concern in the Thurston 2045 survey (Thurston County Community Planning and Economic
Development, 2022), second only to requests to address water issues such as flooding,
landslides, surface and ground water, and preserve wildlife habitat. In the Pacific Northwest,
climate change is expected to manifest in several areas: higher temperatures favor increased
disease, pathogen, and pest occurrences. Warmer temperatures stress agriculture, forests, and
aquatic species. Reduced mountain snowpack and earlier snowmelt from higher temperatures are
likely to increase winter flooding and amplify droughts conditions in the summer. More intense
but less consistent rainfall events enhance the likelihood of floods and erosion during downpours
but means more drying between showers. Extended drying, especially in summer, added to
higher temperatures exacerbates wildfire risks, as evident by repeated record-setting wildfire
outbreaks in the past decade (TRPC, n.d.; USGCRP, 2018; Osterberg et al., 2020). Adapting to
these changes requires a thorough understanding of how our local environment will respond.
More importantly, planners need comprehensive information about all the options available to
address climate change.
The Thurston Regional Planning Council adopted a final mitigation plan in 2020, naming
a suite of strategies to reduce local greenhouse gas emissions to below 2015 levels (45% by 2030
and 85% by 2050). The actions identified in the Thurston Climate Mitigation Plan (TCMP) are
the regional effort to keep global temperatures from rising above 2oC (3.6oF). The plan—
primarily focused on building energy use, transportation, and waste—also includes agriculture,
6

forests, and prairies as potential carbon sinks. In the report, agriculture, forestry, and prairies are
low emission sources (2%, largely fertilizer application and livestock release of methane and
waste) but also have value as potential carbon (C) sinks. The plan calls for reforestation,
afforestation, and increased urban tree cover, as well as preservation of prairies and promotion of
regenerative agricultural practices and education on the benefits of increased organic matter and
water retention in soils (Osterberg et al., 2020). It makes sense to focus efforts on the highest
emissions sectors, but given the options of reducing energy consumption, developing alternative
fuel sources, or sequestering carbon to mitigate climate effects, carbon sequestration may be a
significant, low cost, readily available option (Lal, 2007; Griscom et al., 2017; Bossio et al.,
2020). More importantly, soil carbon sequestration may offer more potential for long-term,
secure storage than forestry projects because of the differences in how the carbon is stored within
the ecosystems. Climate change and anthropogenic pressures place large-scale forest vegetative
carbon storage at risk, whereas carbon sequestered in the soil is less likely to be lost to wildfires
(Bossio et al., 2020; Halofsky et al., 2020). It is likely that the most stable terrestrial carbon sinks
in the future may be grasslands and pasturelands, where up to 80% of the carbon is stored
belowground and is protected from climate effects such as increased wildfire and reduced
productivity resulting from precipitation and temperature fluctuations (Dass et al., 2018; Bossio
et al., 2020; Halofsky et al., 2020). Furthermore, it is possible that grasslands may not have a
saturation point, as continued accumulation of organic matter in every form will lead to higher
soil organic carbon levels in toto (Mayerfeld, 2023).
In the Pacific Northwest, conversation about carbon sequestration often references the
astounding capacity of our native forests, such as the 80 Mg of carbon per hectare stored in our
moist coastal forests (Case et al., 2021). However, this measure is dwarfed by the capacity of soil
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to amass carbon. Across the global carbon spectrum, soil holds 2500 Pg of carbon, including
1550 Pg of soil organic carbon (SOC) and 950 Pg of soil inorganic carbon (SIC), more than four
times the carbon held in all earthly vegetation (Lal, 2004; Weil and Brady, 2017; Gurmu, 2019;
Gutwein et al., 2022). For every ton of carbon stored in the soil, 3.67 tons of CO2 is removed
from the atmosphere (Fynn et al., 2009). Soil carbon sequestration rates are estimated to be
between 300 and 500 Kg of carbon per hectare per year (and as much as 1.0 to 1.5 Mg carbon
per hectare per year for severely degraded soils) (Lal, 2007). Estimates of carbon storage
capacity vary, depending on climate, edaphic factors, vegetation, and management. In humid,
temperate climates, the potential for carbon sequestration can be as much as 1000 Kg carbon per
hectare per year (Lal, 2007). What’s more, simple practices such as grazing at optimal intensity
may increase carbon levels 0.06 Mg per hectare per year on 712 million hectares of global
rangelands and pasturelands; including legumes to 72 million hectares of global pasturelands
may increase storage by a further 0.56 Mg C per hectare per year (Griscom et al., 2017; Bossio et
al., 2020)—an annual sequestration rate of 0.08 Pg C across the globe. Regardless of
sequestration or emission rates, the magnitude of the soil carbon pool indicates that small
changes have large impacts on the global carbon cycle (Fynn et al., 2009; Gutwein et al., 2020;
Bai and Cotrufo, 2022).
Despite growing enthusiasm for high potential soil carbon sequestration, realistic
estimates of soil carbon sequestration capacity are warranted. Claims of 100% offset of
anthropogenic greenhouse gas emissions may undermine more accurate but still substantial soil
carbon sequestration amounts (Giller et al., 2021). Estimates of carbon levels are complicated by
the complexity of soil, external factors such as climate, management, and anthropogenic
pressures, and the responsiveness of different portions of soil to those external influences
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(Kibblewhite et al., 2008; Karlan, Stott, and Mikha, 2021). Limits to carbon sequestration due to
inherent thresholds in the mineral structure of soil, saturation of carbon stocks, and restrictions
resulting from nitrogen deficiencies may restrict soil carbon sequestration to 0.14 ±0.1 Pg C (Six
et al., 2002; Bai and Cotrofu, 2022; Janzen et al., 2022). He et al. (2016) calls into question Earth
Systems Models (ESM), pointing out faults in the models: lack of moisture, temperature, and
other conditional data effects; inconsistent depths; and in situ versus lab-incubation studies. As a
result, the ability of soil to add significant amounts of long-term carbon storage may be
overestimated by a factor of two (40% ±27%) (He et al., 2016). Another study questioning the
positive impact of soil sequestration in managed grasslands indicates that while rangelands and
grasslands in North America and Europe act as carbon sinks, conversion of tropical forests into
pastures and pastures into croplands to produce food for livestock are tipping the balance toward
net carbon loss (Chang et al., 2021). Both studies juggle a great deal of ambiguity, with unknown
warming and CO2 fertilization effects and unpredictable anthropogenic management choices and
pressures complicating soil carbon sequestration capacity estimates.
Uncertainty about climate change effects include soil response to elevated CO2 levels,
which could result in increased net plant productivity (NPP—above ground plant growth) and
increased SOC stocks with attendant drawdown in greenhouse gases (CO2) or in higher
respiration and decreases in SOC stocks (He et al., 2016). In studies of warming effects on soil
carbon, mean average temperature (MAT) most directly influenced soil carbon levels as
increased temperatures spurred microbial mineralization and respiration of carbon dioxide into
the atmosphere, with negative correlation higher in warmer climates than cooler (Lal, 2004;
Reynolds et al., 2015; Yin et al., 2022). When all conditions were equal, however, mean average
precipitation (MAP) became the primary indicator (Reynolds et al., 2015). Higher precipitation
9

in warmer climates spurs carbon emission from soil, which decreases soil carbon storage. In
most cases, warmer temperatures increased NPP, which increases carbon inputs to soil (Reynolds
et al., 2015; Yin et al., 2022). In Massachusetts, drainage class of soil was a significant predictor
of SOC stock (Gutwein et al., 2020), which may be unique to that region or may be a significant
indicator globally. Given projected climate change scenarios for the Pacific Northwest, increased
rainfall in the winter and early spring would likely combine with a warmer spring season to
increase plant productivity without reaching optimal conditions for significant microbial
respiration. However, drier summer weather would likely lead to neutral or negative soil carbon
emissions, resulting in an overall increase in soil carbon storage. Karlan, Stott, and Mikha (2021)
point out the heterogeneity of soils and their different responses to climate and management,
calling for holistic, balanced assessment of the physical, chemical, and biological components of
soil. Kibblewhite et al. (2008) also call for holistic assessment, pointing out the full picture lost
in reductionist approaches. They indicate the adaptability of the biotic components as responsive
to external environment and anthropogenic pressures, while the abiotic elements are less
reactive.
These uncertainties emphasize the spatially distinct and variable results of soil carbon
sequestration studies. Accruing baseline soil carbon levels will permit more accurate estimates of
climate change and management influences on soil carbon storage over time. Consistent
collection methodology, including depth of measurement, laboratory analysis, indicator selection
and interpretation, and input assessment will yield more reliable data for policy makers. Soil
carbon sequestration may not be a permanent solution to climate change, but rather offers shortterm mitigation without negative side effects. Indeed, the ancillary benefits of increased soil
carbon levels—improved water infiltration, enhanced water storage, enriched nutrient cycling,
10

and decreased nutrient and sediment runoff—are motivation enough to encourage practices that
promote soil carbon expansion (Lal, 2013; Giller et al., 2021). Bottom line, whether soil carbon
sequestration achieves the theoretical greenhouse gas offset potential or more modest offsets, it is
one of the few options with virtually no negative consequences and many positive additional
benefits.
To illuminate in more detail how soil carbon sequestration occurs and how management
affects carbon levels in pasturelands, a brief explanation of soil components is followed by a
look at measurement standards, biomes in Southwest Washington, and finally how pasture
management affects soil and ultimately carbon levels within pastures.
2.2 Introduction to Soils
It is necessary to understand how soils work to recognize the relationship between soil
and climate change mitigation. Soil is an extraordinarily complex system that influences nearly
every aspect of terrestrial life. The physical, chemical, and biological components of soil act
together to create growth media for plants, animals, and humans, and is one of the most diverse
ecosystems on earth (FAO, 2015). Healthy soils provide “continued capacity of soil to function
as a vital living ecosystem that sustains plants, animals, and humans” (NRCS, n.d.; Weil and
Brady, 2017). The inverse, unhealthy soils, restricts sustained growth and well-being, and
therefore require attentive care and management.
2.2.a Soil properties
2.2.a.1 Soil physical properties
Soil is one of the most overlooked yet valuable natural resources on earth. Ubiquitous
and seemingly inexhaustible, the rate at which natural soil forms—between 0.017mm and
0.083mm per year from a combination of parent minerals, vegetation, climate, and time—is
11

beyond the human time scale for regeneration (Montgomery, 2007; Weil & Brady, 2017). The
physical components of soil provide a framework for soil biotic and chemical interactions
controlling soil functions. Soil texture, or particle size distribution, from clay (fine) to silt to sand
(coarse) determines the ability of soil to attract and hold water and nutrients, which influences
soil carbon content. Soil texture influences soil aeration, drainage, erodibility, compactibility,
and plant root and microbial movement through soil (Weil & Brady, 2017).
While some aspects of soil physical properties are unchangeable—sand will remain
sand—biotic interaction influences processes such as soil aggregate formation. Plant roots and
fungal hyphae bind soil particles, bacteria and fungi organically affix soil, and soil fauna—often
earthworms—mold soil aggregates (Weil & Brady, 2017). Finer textured soils such as silt loams,
clays, and clay loams tend to have lower bulk densities—a measure of soil weight over unit
volume—than sandy soils because their aggregation, especially with organic matter, makes more
soil pores. (Reganold, 1993). Soils with high bulk density and compacted subsoils have reduced
biological activity because water and air movement, as well as root growth and faunal
movement, through the soil are restricted (Reganold, 1988; Weil and Brady, 2017). Bulk density
is negatively correlated with soil organic carbon, is influenced by management (especially
grazing), and must be calculated to estimate soil organic carbon stocks (Van Haveren, 1983;
Sakin, 2012; Li et al., 2017).
2.2.a.2 Soil biological properties
Soil microorganisms such as bacteria, fungi, archaea, nematodes, worms, and arthropods
contribute function in soil through oxidation of plant carbon compounds (root exudates or leaf
litter), nutrient release, compound synthesis, or protection of organic materials (Schjonning et al.,
2004; Weil and Brady, 2017). Soil microorganisms exchange plant-derived carbohydrates for
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water, nitrogen, phosphorus, potassium, and other nutrients while respirating carbon dioxide
back into the soil and atmosphere (Ladoni et al., 2015; Li et al., 2017; Weil and Brady, 2017).
The synchrony between these exchanges is very tight, and there is little extra carbon dioxide
released into the air or excess nutrients left in the soil. In a symbiotic dance, a variety of plant
materials supports diverse soil microbial biomass, who in turn increase plant phosphorus uptake,
enhance nitrogen availability (Mader et al., 2002; Naylor et al., 2020; Giller et al., 2021), and
balance nutrient mineralization, particularly nitrogen (N) with plant uptake cycles. In natural
ecosystems, these events are also choreographed with seasonal precipitation and temperature
changes. Soils with higher soil organic matter content have much higher biodiversity of bacteria,
mycorrhizal fungi, protozoa, nematodes, earthworms, and arthropods between and within species
(Mader et al., 2002; Esperschutz et al., 2007; Crowder et al., 2010; Naylor et al., 2020). These
diverse populations perform a host of functions, nitrogen fixation, macropore tunnel creation,
and microaggregate formation (Schjonning et al., 2004).
Soil organic matter (SOM) includes living and deceased plants and animals and is
estimated to be 50-58% soil organic carbon (Pribyl, 2010; Weil and Brady, 2017; Gurmu, 2019).
The degree of SOM in soil affects soil capacity to hold water. Hudson (1994) found that silt
loams containing 4% organic matter had more than twice the available water content than a silt
loam with 1% organic matter. This contradicted previous beliefs that greater amounts of organic
matter increased plant wilting points and effectively reduced plant available water. The increase
of soil organic matter increases water infiltration capacity and water storage capacity, as much as
144,000 liters of water/ha for a 1% increase in soil organic matter (Sullivan, 2002, as quoted by
White, 2020).

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2.2.a.3 Soil chemical properties
Chemical properties of soil refer to processes mediated by inherent physical soil
characteristics, organic inputs, environmental conditions such as moisture or temperature, and
biological activities. The ratio between carbon and nitrogen, determined by plant input and
microbial processing, also affects the productivity and diversity of the microbial biomass,
because diverse microbiota have different input requirements. (Weil and Brady, 2017). A
balance of organic materials of differing maturity creates deeper, more diverse pools of carbon
materials.
The variety of materials creates higher diversity in microbial biomass, with fresher
materials spurring rapid processing and nitrification by some microorganisms and older, more
stable materials processed by other microorganisms. (Clark et al., 1997; Mader et al., 2002;
Wachter, 2019). Lower C:N ratios found in more labile carbon matter (i.e., proteins, enzymes,
and carbohydrates from fresh plant materials) spur rapid nitrification and thus high respiration
rates by resource-acquisitive organisms that process labile organic matter quickly, reducing soil
organic matter levels and soil carbon stocks. Higher C:N ratios found in more mature plant
matter (lignin and cellulose) create nitrogen deficiencies and slow respiration rates as k-strategist
organisms more slowly process recalcitrant carbon, reducing CO2 emissions and resulting in
higher SOC storage. As microbes process carbon, portions of the organic material become
unavailable—recalcitrant—to the microbial population or to plant uptake in a process called
humification. The tiny fragments of carbon matter are adsorbed or chemically bonded to the
smallest soil particles, what we call sequestered soil organic carbon (Weil and Brady, 2017).
This adsorption increases the cation exchange capacity (CEC) of the soil. Increased CEC
capacity in soil plays many roles in soil health. Micronutrients needed for plant and

14

microorganism health are held on cation exchange sites. Increased CEC found at higher soil
organic matter levels increases soil fertility. Hydrogen cations (H+) released from microbial
respiration can also be held on the cation exchange site, which has direct implications for the pH
level of the soil. While increased H+ released from elevated microbial respiration typically leads
to lower pH, higher CEC found in soil organic matter expands the number of cation exchange
sites, which enhances the pH buffering capacity of the soil, enhancing fertility and production
(Franzluebbers, 2010). This is an example of how increases in SOM matter (increased SOC)
provides ancillary benefits.
Additionally, soil microbial diversity influences soil pathogen suppression. Soil pH can
support or inhibit microorganisms at differing levels, affecting the uptake of micronutrients by
soil biota. The dominance of particular communities of soil microorganisms influences the
ability of others to affect soil and plant health. Greater diversity of microbial biomass increases
evenness within the populations and creates functional redundancy as well as increases
suppressiveness of the soil (Crowder, 2010).
2.2.b Soil as an ecosystem
2.2.b.1 Ecosystem services
Soil provides many ecosystem services: water filtration and storage; pollutant
attenuation; flood regulation; habitat and biodiversity; nutrient cycling; provision of food, fiber,
and fuel; provision of construction materials; carbon sequestration; and climate regulation.
Moreover, soils with high SOC content typically have good soil structure and efficient nutrient
cycling, creating natural CH4 and N2O sinks (Lal, 2013). Over the past forty years, inimitable
and irreplaceable soil ecosystem services have gained increasing attention (Kibblewhite et al.,
2008; Lal, 2015; Adhikari and Hartemink, 2016), but not enough to make soil health and
15

preservation a top priority. The essential nature of a healthy, diverse soil microbial population is
echoed in the functions and services provided. In this most diverse ecosystem—25% of earth’s
biodiversity—thousands of bacterial species, hundreds of fungal and insect species, and tens of
mites and nematodes, and several vertebrates and earthworms consume and process plants,
organic matter, and even pollutants. They increase soil fertility, enhance water and nutrient use
efficiency, support plant production, and moderate the carbon cycle (FAO, 2015). Without these
species and their functional diversity, many ecosystem processes cease to function.
2.2.b.2 Role in climate change mitigation
Globally, the largest pool of organic carbon, 2344 Pg, exists in soil with conservative
estimates of lost SOC ranging from 42-78 Pg due to land clearing, oxidation of organic matter,
and erosion (Lal, 2004; Franzluebber, 2010; Stockman et al., 2012; Zomer et al., 2017).
Cultivation of soils has led to the release of 50-70% of soil carbon—10 to 30 Mg/ha, depending
on how the land has been used and soil type—creating the opportunity for increased SOC
sequestration (Lal, 2004; Lal, 2013: Zomer et al., 2017). By comparison, carbon losses from soil
from land use conversion (136 ±55 Pg) and SOC depletion (214±67 Pg) are greater than
terrestrial carbon losses due to fossil fuel consumption (270±30 Pg). A single petagram (Pg) soil
carbon is approximately equal to 0.47 ppm or mg/kg of atmospheric CO2 (Lal, 2013). Replacing
depleted soil organic carbon through carbon sequestration has substantial potential for drawing
down atmospheric CO2 (Lal, 2004; Lal, 2007; Aguilera et al., 2016). Land systems, including
pasturelands, are a major sink for CO2 in live organic matter, 100-1000kg C/ha in humid and
cool climates. (Lal, 2004).

16

2.2.b.2.i Carbon sequestration
Soil organic carbon sequestration is the method by which atmospheric CO2 is conveyed
through plant photosynthesis into the soil as plant matter, residues, or exudates that become part
of the soil organic matter for some amount of time, days to millennia (Olson, 2010). The plant
inputs are consumed by soil biota who either utilize and respirate a portion of the carbon (CO2)
back into the atmosphere or break the organic carbon down further. The senesced bodies of the
initial biotic carbon consumers are consumed in turn by other biota, who repeat the process. The
degree of soil carbon sequestration is dependent on endogenous factors: soil texture, parent
material, internal drainage, biotic, and chemical factors. It is also dependent on exogenous
factors: land use, disturbance, vegetation, climate, and mineralogy (Lal, 2013; Zomer et al.,
2017).
Other factors influencing soil carbon storage include the source of input—root associated
carbon is more stable partly because greater chemical recalcitrance, partly because of physical
depth and µm scale protects from mycorrhizae and root-hair activity (Stockman et al., 2012),
while leaf litter is more labile (Stockman et al., 2012) Current research indicates that particulate
organic matter (POM) is more labile and mineralizable, with a mean residence time (MRT) of
less than 10 years than mineral associated organic matter (MAOM) whose MRT is 10s to 100s of
years (Bai and Cotrufo, 2022). Saturation of MAOM and increase in decomposition (loss of
SOC) may reduce realistic sequestration rates to 0.14 Pg C/year in cropping systems, but
perennial vegetation, such as in pasturelands, could increase SOC levels (Janzen et al., 2022;
Conant et al., as quoted in Mayerfeld, 2023). SOC stocks also increase as mean average
temperatures (MAT) decrease (Stockman et al., 2012), while laboratory studies of warming
indicate SOC loss, particularly in the whole-soil profile (3.1 Pg C/year with 4oC) (Hicks Pries,

17

2017). Vegetation coverage and input influence SOC stocks, with perennial pastures offering
great potential year-round growth and manure deposition (Franzluebber, 2010). Potential for
carbon sequestration is highest in North America (0.60 to 1.22 t C/ha/year/0.17-.35 Pg C
annually (Zomer et al., 2017) particularly in temperate climates where NPP is high and microbial
respiration is low. Potential for carbon sequestration is higher at higher latitudes, and highest
globally in the United States (Zomer et al., 2017).
Grasslands in low temperature, high precipitation (greater than 500mm annually) zones
have the highest initial SOC stocks globally, due to nutrient limitations and low pH hindering
microbial activity. Grazing stimulates growth in grasslands, particularly for C4 grass species,
which can increase SOC storage; however, pugging (animal treading on soil) can spur microbial
decomposition and loss of SOC (Abdalla et al., 2018). With MAP ranging from 1300mm to more
than 2400mm and MAT from 47oF to 51oF in Southwest Washington (NOAA-NCEI, n.d.),
carbon stocks should be relatively high.
2.2.b.2.ii Flood and drought resistance/resilience
As competition for water resources increases, increasing soil water capacity has
substantial benefits not only for producers, but also for all water users. Hudson (1994) found that
silt loams containing 4% organic matter had more than twice available water content than a silt
loam with 1% organic matter, contradicting previous beliefs that greater amounts of organic
matter increased plant wilting points and effectively reduced plant available water. The increase
of soil organic matter increases water infiltration capacity and water storage capacity, as much as
144,000 liters of water/ha for a 1% increase in soil organic matter (Sullivan, 2002, as quoted by
White, 2020). For example, a study in New Zealand found a 1% increase in SOC had a positive
effect on non-readily available water, increasing the water holding capacity of dry pastures (Fu et
18

al., 2012). Given projected precipitation changes in the coming years in Southwest Washington,
having water available under drought conditions is valuable.
2.2.c Soil analysis
One of the factors discouraging adoption of soil carbon sequestration as a climate change
mitigation pathway is the difficulty assessing soil in spatially and climatically heterogeneous
conditions. Soil is a dynamic, responsive system that reacts to management and exogenous
influences in varying ways and complicates efforts to quantitatively assess soil. Extensive review
by scientists, agricultural producers, and technical service providers resulted in four benchmarks
for selecting indicators: effectiveness, accessibility, consistency, understandability for
management applications (Karlan, Stott, and Mikha, 2021). Effectiveness evaluates
responsiveness to management changes and applicability of the criteria assessed. Ease of use and
cost effectiveness determine accessibility, while consistency refers to uniform results for the
same measurements. Understandability is perhaps the most difficult to standardize, because it is
more nuanced for local ranges, results, management practices and outcomes. In this study, the
selection of which indicators to measure, which methods to use collecting samples, which
laboratories and laboratory tests to employ, and which interpretations to apply are based on a
synthesis of soil health frameworks.
USDA-ARS Greenhouse Reduction through Agricultural Enhancement network
(GRACEnet) protocols examine agricultural soil C stocks related to greenhouse gas
sequestration to examine agricultural system influence on SOC (Karlan, Stott, & Mikha, 2021).
The GRACEnet data entry template (DET) stores site descriptions (latitude, longitude,
topographical descriptions—slope, flat, etc.); soil characterization (taxonomy of most common
soils at the site, soil type, texture, typical pH, bulk density, and soil nutrient information—total C

19

and N, organic and inorganic C, NO3, NH4); research design details (number and size of sites,
replications, and treatments, depth of sampling, implements for collection, whether the sample is
a composite); climate (mean annual temperature and precipitation, weather for at least the last
two years, and possibly information about the nearest weather recording station); soil
management (amendments—fertilizer/pesticides/organics with rates and application details, land
use history, irrigation, drainage); and livestock management (animal species and class, stocking
rates, duration of grazing, frequency of rotational grazing, manure management). I did not use
this framework because the components for study design and treatment were not applicable.
Other frameworks include the Soil Management Assessment Framework (SMAF) from
USDA-ARS and NRCS and Comprehensive Assessment of Soil Health (CASH) from Cornell
University. Both assessments examine soil function as influence by management. SMAF looks at
13 factors on a scoring curve: wet aggregate stability (WAS), bulk density, water-filled pore
spaces (WFPS), available water capacity (AWC), electrical conductivity (EC), pH, sodium
adsorption ratio (SAR), extractable P and K, SOC, microbial biomass C (MBC), potentially
mineralizable N (PMN), and β-glucosidase activity (BG). Most of the scoring curves are
calibrated for the North American Great Plains region, from Canada to TX, and internationally.
A long-term study in Arkansas used SMAF assessments to assess soil across five different
categories with at least one indicator each for physical, chemical, and biological soil properties to
determine the impacts of pasture management on soil (Amorim et al., 2020). My study in
Southwest Washington pasturelands echoes the Arkansas study with examination of pH and
extractable P and K (chemical), bulk density (physical), and SOC (biological).
The CASH assessment was developed using SMAF concepts but sought greater
sensitivity and more rapid processing (autoclave-citrate extractable (ACE) proteins substitute for

20

PMN and permanganate-oxidizable carbon (POXC) for MBC). CASH has been used in Kenya,
Pakistan, Colombia, North Carolina, and New York, and usually indicates that easily digestible
types of C and N (POXC, ACE protein) and WAS respond readily to management changes,
making them good indicators of soil biological and physical properties (Karlan, Stott, & Mikha,
2021). However, as in the case with the SMAF structure, CASH is not calibrated for a wide
geographical area. In both systems, site-specific details such as soil type and texture,
environmental conditions, research protocols, and management practices were important factors
and should be considered in assessing soil quality. I used portions of the CASH/SMAF
frameworks for my study, which is also the framework for the ongoing Washington State
Department of Agriculture State (WSDA) of the Soils assessment (WSDA, 2022).
Consistency in sampling and testing impacts soil assessment results. Protocols for
handling and preparation of samples, equipment calibration, decisions determining which tools
and tests to utilize, and proficiency at following established procedures vary. Even research
design decisions influence the level of information relayed in a single experiment, as changes in
soil health due to management practices in temperate climates can take 3-5 years, maybe as
much as 10 years, to detect (Franzluebber, 2010; Karlen, Stott, & Mikha, 2021; Chase, 2022).
Furthermore, differences between field tests and laboratory tests in both level of data revealed
and timeliness of results require careful consideration of goals. Soil biological properties are the
most dynamic and difficult to assess but are the drivers of most soil functions. USDA NRCS and
ARS initiated a national database review to select actionable soil indicators. In data evaluated
from 38 states, 60% reported SOC as the primary indicator of soil health (Karlen, Stott, &
Mikha, 2021). The SOM and SOC results from each site are the primary data for my study.

21

2.2.c.1 Physical
There are many methods to measure bulk density as a common indicator of soil physical health.
One of the most common and inexpensive is the core method. In comparison to the clod method,
excavation method, radiation method, and regression method, core method shows no significant
difference at varying depths, does not require expensive equipment or knowledge, and is less
susceptible to operator error. Use of a 100cm3 steel core is often the most reliable, as smaller
cores impact the soil structure (Al-Shammary et al., 2020). The relationship between SOC and
SOM are strongly positively correlated, although the ratio may differ between soil types and
depth. On the other hand, bulk density is strongly negatively correlated to SOC (Sakin, 2012).
Bulk density as an indicator reveals information about soil physical health such as soil
aggregation and available water content (Lal, 2013), factors that heavily influence SOC accrual.
2.2.c.2 Biological
Soil organic matter exerts an acute influence on the functions of soil despite its small
proportion of soil, affecting nutrient cycles, water processes, plant growth, and pollution
management, and needs to be at least 1.1-1.5% by weight of soil to provide minimal function
(Gurmu, 2019; Lal, 2015). Although SOM and SOC are preferred biological health indicators,
the influences of physical and chemical properties can affect SOM and therefore SOC levels,
sometimes invalidating its worth as an indicator. In a 15-year study of five management practices
using the SMAF framework, researchers found little differences in SOM or soil degradations
except phosphorous concentrations (attributed to long-term inputs from livestock), and instead
found soil health was best described by soil fertility (Amorim et al., 2020). Measurement of
short-term mineralizable carbon (SMC) is recommended for assessing management-induced
changes in SOC is recommended, rather than particulate organic carbon (POC) or Total organic
22

carbon (TOC). TOC requires a substantial difference (80% with an acceptable type II error of
0.20) and is slow to respond to management changes, and POC variability is too great, at several
soil depths. In contrast, SMC shows rapid response to management changes and lower
variability; the addition of topographical and soil information increases the statistical power of
tests and chance of detecting changes, especially in large fields where heterogeneity is large
(Ladoni et al., 2015). As a reliable, rapidly obtained, economically feasible assessment of
biological soil properties, I used SOM as the biological indicator in my tests.
2.2.c.3 Carbon sequestration stabilization
Native soils have measurable SOC levels that may be constrained by exogenous or
endogenous limitations, such as temperature-induced rapid decomposition or water limitations.
Agricultural or other management can overcome inherent soil limitations (for instance, by
correcting nutrient deficiencies) impacting net primary production (NPP) inputs to soil carbon
levels. However, despite substantial carbon sequestration potential in soil, conflicting analysis
about limits of soil carbon sequestration cloud the issue. Protection of SOM through several
mechanisms (physical protection in silt and clay, microaggregation occlusion, and biochemical
protection) can be maximized, but not exceeded. Sequestration of SOC follows the path from
unprotected, active carbon found in light fraction and particulate organic matter (POM) to the
increasingly unavailable slow and passive fractions—mineral associated organic matter
(MAOM) hidden in microaggregates, adsorbed to soil particles, and biochemically transformed
into stable SOC pools (Six et al., 2002). Furthermore, limits to soil carbon sequestration are tied
to plant productivity and nutrient balances. For every 24 g of carbon processed by soil microbes,
an average of 1 g of nitrogen is required (Weil and Brady, 2017; Janzen et al., 2022). Nutrient
imbalances can be addressed by increased nitrogen inputs (fertilizer or manure applications) or
23

reduced nitrogen losses (legume inclusion). MAOM, 55% originating from root exudates and
root tissues, is nitrogen dependent and limited by the textural composition of the soil. POM, on
the other hand, is not dependent on nitrogen for microbial processing and may continue to
accumulate. POM is less stable and subject to land management and environmental factors such
as temperature and precipitation (Bai and Cotrufo, 2022), but it does not have an inherent upper
limit (Conant et al., 2017 as quoted in Mayerfeld, 2023). Grassland topsoil contains 50-75%
SOC in MAOM, and has a microbial necromass average of 50%, larger than agricultural or
temperate forest soils, which indicates a more stable SOC pool in grasslands (Bai and Cotrufo,
2022). The good news for climate mitigation via soil carbon sequestration is that SOC levels—in
either fraction—are far from saturation today.
2.2.d Soil Texture
Soils in the Southwest Washington sites in my study are grouped by the Natural Resource
Conservation Service (NRCS) Ecological Site Descriptions (ESD) of Puget Lowlands Forest,
Puget Lowlands Moist Forest, Puget Lowlands Wet Forest, Puget Lowlands Riparian Forest, and
Puget Lowlands Prairie (Table 1). Nearly all these soils were formed by glacial and volcanic
activity, with some alluvial influences. Everett-Spanaway complex, Everett very gravelly sandy
loam, Kapowsin stony loam, and Alderwood gravelly sandy loam are the Puget Lowlands Forest
(PLF) soils in the study. While they have a high amount of sand, ranging from 42% to 68%, they
also have high levels of silt, 25% to 37% (Figure 1). The Puget Lowlands Forest soils have
relatively low levels of clay, 5% to 8% (except Kapowsin, with 20% clay). The Puget Lowland
Moist Forest (PLMF) soils are Doty silt loam, Melbourne loam, Yelm fine sandy loam, and
Cathcart gravelly loam. These soils have higher levels of clay, 10% to 28%, and silt, 22% to
53%. As the moisture level increases to the Puget Lowlands Wet Forest (PLWF) soils—Skipopa

24

silt loam, Everson clay loam, and Bellingham silt loam—the clay levels are much higher, 18% to
33%. Sand hovers around 30%, and silt levels range from 32% to 54%. Soils in the Puget
Lowlands Riparian Forest (PLRF) are Chehalis silty clay, Puyallup silt loam, Elma-Fordprairie
Complex, and Maytown-Chehalis-Rennie Complex. Silt levels are highest among the Puget
Lowlands Riparian Forest soils, ranging from 47% to 66%. Clay is also higher in the 9% to 45%
range. The amount of sand in PLRF soils is lower, 7% to 38%. The Puget Lowlands Prairie
(PLP) soils, Spanaway-Nisqually complex and Spanaway gravelly sandy loam, are highest in
sand content, 68% to 82% and unsurprisingly low in clay, 4% to 8%, given the glacial outwash
source of these soils (WSS, n.d.).
Table 1.
Soil textures of Southwest Washington Ecological Site Descriptions
Clay
%
Sand
%
Silt %

PLP
Mean: 7.1%
Median: 8%
SD: 1.29%
Mean: 71.1%
Median: 68.2%
SD: 4.81%
Mean: 21.8%
Median: 23.5%
SD: 3.95%

PLF
Mean: 8.7%
Median: 5.1%
SD: 6.53%
Mean: 59%
Median: 62.9%
SD: 9.5%
Mean: 32.3%
Median: 32.1%
SD: 4.50%

PLMF
Mean: 17.6%
Median: 15.9%
SD: 8.51%
Mean: 43.7%
Median: 41.4%
SD: 16.63%
Mean: 38.8%
Median: 39.4%
SD: 13.14%

PLWF
Mean: 24.8%
Median: 24%
SD: 6.76%
Mean: 28.7%
Median: 27.3%
SD: 3.76%
Mean: 46.1%
Median: 49.2%
SD: 9.73%

PLRF
Mean: 24.1%
Median: 20.9%
SD: 15%
Mean: 22.8%
Median: 22.9%
SD: 14.91%
Mean: 53.1%
Median: 50.6%
SD: 0.50%

Note. PLP = Puget Lowlands Prairie, PLF = Puget Lowlands Forest, PLMF = Puget Lowlands
Moist Forest, PLWF = Puget Lowlands Wet Forest, PLRF = Puget Lowlands Riparian Forest.
Soil texture data is from Web Soil Survey weighted averages (NRCS, n.d.), descriptive statistics
from R Studio.
2.3 Biomes in Southwest Washington
2.3.a Native Forest
Volcanic soils scoured by glaciers provide a deep, rich seed bed for native forests,
(Pojar and MacKinnon, 1994). Soil heterogeneity, common in Southwestern Washington, ranges
from the Scatter-Fordprairie-Roundtree complex to Melbourne loam to Buckpeak silt loam
25

(NRCS, n.d.). Large evergreen trees such as Douglas fir (Pseudotsuga menziesii), Western
hemlock (Tsuga heterophylla), Western redcedar (Thuja plicata), and Pacific madrone (Arbutus
menziesii) are complemented by large deciduous trees, including Red alder (Alnus rubra),
Bigleaf maple (Acer macrophylla), and Black cottonwood (Populus balsamifera). Understory
shrubs include salal (Gaultheria shallon), several species of huckleberry (Vaccinumium spp),
rhododendron (Rhododendron macrophyllum), elderberry (Sambucus racemose), snowberry
(Symphoricarpos albus), oceanspray (Holodiscus discolor), Indian-plum (Oemleria cerasiformis),
and a host of berries (Rubus spp) (Pojar and MacKinnon, 1994). All these native species are
well-adapted to the wet winter, dry summer, temperate climate typical of the Pacific Northwest,
although long-term adaptability to climate changes is unknown. Fire-opportunists like Douglas
fir and red alder have encroached on previously open grasslands, expanding forested lands
(Dunwiddie et al., 2014) and giving credence to those species’ adaptability to non-forest
environmental conditions. The highly productive forests, due to high precipitation levels and
relatively nutrient rich soils define much of the vegetation of Southwestern Washington,
particularly Lewis, Mason, and Grays Harbor counties (NRCS, n.d.). In fact, potential carbon
sequestration by Pacific Northwest forests is considered very high—often more than 80Mg per
hectare, compared to 40-60Mg for Midwestern forests and 60-80Mg in Northeast forests—
contained in the aboveground tree matter (Case et al., 2021).
2.3.b Native Prairies
Native grasslands, or prairies, exist in the Pacific Northwest because of fire. Drier, welldrained soils supported native perennial grasses and were maintained by anthropogenic burning
(Pojar and MacKinnon, 1994). Indigenous communities burned to retain open spaces and
cultivate berries, nuts, vegetables, and forage for ungulate game. Suppression of fire has

26

transitioned open grasslands to woodlands and then forests in many areas in Southwest
Washington (Pojar and MacKinnon, 1994). Formed from glacial outwash, native prairies are
well-drained and often marked by low organic matter, although some have improved water and
nutrient characteristics from wind-deposited silt. Common prairie soils in Southwest Washington
are Spanaway gravelly sandy loam and Nisqually loamy fine sand (NRCS, n.d.).
Typical native vegetation includes Roemer’s fescue (Festuca roemeri), Common camas
(Camassia quamash), Western buttercup (Ranunculus occidentalis), Spring gold (Lomatium
utriculatum), Slender cinquefoil (Potentilla gracilis), Paintbrush species (Castilleja spp), Lupine
species (Lupinus spp), Menzies’ larkspur (Delphinium menziesii), Chocolate lily (Fritillaria
lanceolata), and Garry oak (Quercus garryana). These species are perennials well adapted to the
soils and climatic conditions of the Southwest Washington native prairies. Common invasive
plants dominating Southwest Washington prairies are Scotch broom (Cytisus scoparius),
Himalayan blackberry (Rubus armeniacus), Rat-tail fescue (Vulpia myuros), and Tansy ragwort
(Senecio jacobaea) (Pojar and MacKinnon, 1994). Exclusion of fire from the native prairies and
the adaptive and aggressive nature of the invasive species make them a significant threat to the
native species.

Belowground allocation—30% to 50%—of carbon in grassland species, higher levels of
arbuscular mycorrhizal fungi found in grasslands, and better water use efficiency in grasslands
systems, especially those dominated by annual species, result in increased MAOM fractions
(Bachelet et al., 2011, Dass et al., 2018; Bai and Cotrufo, 2022). Comparing carbon stocks in
temperate grasslands and forests, the German Advisory Council on Global Change estimated 9
Pg of carbon in vegetation and 295 Pg in soil (304 Pg total) for grasslands versus 59 Pg in

27

vegetation and 100 Pg in soil (159 Pg total) for forests (German Advisory Council on Global
Change, 2000, as quoted by Bachelet et al., 2011). Although soil carbon storage levels are
highest when MAT is less than 0oC and MAP is 900mm to 1000mm—Southwest Washington
MAT ranges between 8oC and 10oC and MAP between 1300mm and 2400mm—the potential for
significant SOC storage remains high in the region (NOAA-NCEI, n.d.; Bai and Cotrufo, 2022).
Prairies have the potential to mitigate climate change as a carbon sink, if they are not degraded
through poor management practices that promote erosion or (in a pastoral setting) overgrazing
(Bachelet et al., 2011; Chang et al., 2021). Prairie restoration may boost species diversity,
enhancing soil biodiversity and soil functions, resulting in improved soil carbon stocks—430
Kg/hectare of carbon—and ancillary benefits such as increased water storage (Matamala et al.,
2008; Bachelet et al., 2011). Due to land use change, human development, and cessation of
Indigenous fire management, approximately 97% of Southwestern Washington prairies have
been lost (Dunwiddie et al., 2014). Restoration of native prairies may increase SOC stocks as
much as 28% over cultivated or degraded soils (Kampf et al., 2016). Preserving and restoring the
remaining native prairies provides another avenue to increase soil carbon sequestration in
Southwest Washington.

2.4 Pasturelands
Pasturelands—managed and grazed habitats—in Southwest Washington are most often
former prairies, particularly those with reduced fertility and water-holding capacity, although
some evolved from cleared forest lands. Pasturelands worldwide constitute roughly 40% of land
and 70% of agricultural acreage (Abdalla et al., 2018). In Southwest Washington (Thurston,
Lewis, Mason, and Grays Harbor counties), there are nearly 25,000 hectares of pasturelands,
which is 19.9% of agricultural land (USDA NASS, 2017). Some native species persist in
28

pasturelands, while introduced forage species such as Tall fescue (Schedonorus aurundinaceus),
Tall oat grass (Arrhenatherum elatius), Sweet vernal grass (Anthoxanthum odoratum), Timothy
grass (Phleum pratense), Field meadow-foxtail (Alopecurus pratensis), Brome species (Bromus
spp), Kentucky bluegrass (Poa pratensis), Colonial bentgrass (Agrostis capillaris), Orchard grass
(Dactylis glomerata), Thistle species (Cirsium spp), Clover species (Trifolium spp), and Vetch
species (Vicia spp) are the dominant species. The density of nonnative species and competing
goals for livestock management may impact the functioning of pasture ecosystems, creating a
distinct difference from native prairies. Comparison of soil carbon levels between the two
systems may illustrate management practices that can optimize soil carbon sequestration.
2.4.a Role in carbon cycle
Carbon stocks in pasturelands are primarily determined by pasture plant dynamics—most
C is stored in belowground biomass and deposited in the rhizosphere, and roots grow and
senesce rapidly (Lorenz, 2018)—and influenced by environmental factors. Precipitation
increases primary vegetation growth, increasing soil carbon, and temperature increases
decomposition, reducing the soil carbon pool. Vegetative production and decomposition are
highest in the 25oC to 35oC range when soil moisture is between 50% to 80% of water-filled pore
space. Decreases in temperature hinder decomposition more than plant growth, as does either
soil saturation or excessive dryness (Schnabel et al., 2000). Mean temperatures in Southwest
Washington are well below that threshold for most of the growing season (US Climate Data,
n.d.), which indicates continuous plant production without attendant decomposition and SOC
loss.
Through the process of photosynthesis, plants combine energy from the sun and carbon
dioxide from the air to create energy. Grasses store their long-term energy in the bottom three to

29

six inches of their stems, whereas herbaceous species like clover (Trifolium spp), alfalfa
(Medicago sativa), birdsfoot trefoil (Lotus corniculatus), and legumes store their reserves in their
roots, rhizomes, and crowns (Shewmaker & Bohle, eds., 2010). When growth is disturbed by
grazing, the plant regrows from meristematic tissues—growth points, or nodes—in stems, roots,
and leaves. Apical meristems may be vegetative or reproductive at the growing tip of a plant,
either shoot or root. Growth from vegetative apical meristem can persist until it is eaten, dies due
to natural causes (overshading or old age), or changes to reproductive stock, where it stops shoot
growth. Apical meristems also control two other meristematic tissues: intercalary and axillary
meristems. Found at the base of leaves, grass blades, or blade sheaths, intercalary meristematic
tissues drive extension of tissues and can be dormant at times. If the apical meristem is
defoliated, an active intercalary meristem may generate new growth until the leaf is completely
grown. The axillary meristem, also called the tiller bud, crown bud, or basal bud, is frequently
inert until the apical meristem ages into reproductive stage or death or until it is removed,
allowing the axillary meristem to become the apical meristem (Shewmaker & Bohle, eds., 2010),
a process of new shoot formation called tillering.
Tillering varies among species, but in general increases the density of both roots and
shoots as new tissues replace dead (Shewmaker & Bohle, eds., 2010). This raises the amount of
carbon input to the soil as the senesced material is replaced by living. Related to the amount of
plant growth, and therefore carbon inputs, is the seasonal growth cycle. Typical growth is highest
in late spring and drops as temperatures rise. Additionally, reduced soil water and even lower
soil nitrogen slow growth (Shewmaker & Bohle, eds., 2010). Maximizing regrowth is therefore
dependent on management of herbivory in concert with growth of pasture vegetation.

30

2.4.b Management practices
In pastures, the soil-plant-animal relationship is managed by producers who control the
number of livestock on a pasture and the length of time they are allowed to feed. While we have
looked at the relationship between soils and plants in previous sections, the relationship between
plants and animals—herbivory—bears examination. Because grazing animals may consume 2075% of the aboveground production, their management directly impacts the carbon cycle in
pasturelands (Taboada et al., 2011; Kampf et al., 2016; Abdalla et al., 2018). Vegetative
production increases with precipitation, creating a larger effect on aboveground fodder
consumption in more humid areas such as Southwest Washington (Taboada et al., 2011; Lorenz,
2018). Root effects from grazing are generally positive. Grass with 50% of its foliage removed
loses only 2%-4% of its root growth, indicating that belowground functions are largely
unhindered by grazing (Crider, 1955). Overall, grazing reduces carbon inputs from aboveground
biomass—leaf litter—to POM because of animal ingestion. In fact, decomposition by microbial
biomass of labile carbon is reduced when above ground inputs from leaf litter are decreased and
less digestible root carbon, twice as slow to decompose as leaf carbon, is the primary source of
microbial energy. The increase of the MAOM carbon pool from root carbon and reduction of
POM inputs increases SOC stocks. Grazing affects the composition and function of the microbial
pool by altering the quantity and quality of the carbon input they receive. Partially processed
carbon in feces are high quantity and quality inputs with limited distribution. Above ground,
grazing in productive pasturelands increases leaf nutrient content, increasing litter quality and
decomposition, while below ground, carbon allocated to root exudation increases, initiating the
microbial activity-nutrient feedback cycle in the root area that supports plant growth. (Taboada et
al., 2011; Naidu et al., 2022). Plant community structure influences microbial activity when the

31

types of plants are more readily digested, such as invasives that accelerate the carbon cycle or C4
grasses whose daily growth is 19% to 88% higher than C3 grasses. In areas of high vegetative
growth, stimulation of above and below ground productivity—by grazing, for example—can
offset the loss of litter carbon inputs. (Taboada et al., 2011; Lorenz, 2018; Naidu et al., 2022).
Finally, grazing affects the microbial community composition, although studies contradict the
specific effects. Taboada et al. (2011) suggest grazing shifts the microbial community
composition toward a faster, bacterial dominated population, rather than a slower, fungal
dominated one. In contrast, Naidu et al. (2022) suggest grazing has a stabilizing effect on soil
carbon because it extends the stoichiometric coupling between nitrogen and carbon.
Managers of grazing systems primarily focus on the number of animals in a pasture (the
stocking rate) to achieve financial, environmental, and other objectives. Management methods
control the length and timing of grazing, the amount and type of forage consumed by different
animals, and how much time each pasture is grazed (Shewmaker & Bohle, eds., 2010; Moore et
al., 2019). The type of management practiced depends on climate, geography and topography,
inputs (seeding, fertilizer, irrigation), management intensity, which part(s) of the year the pasture
is utilized, animal type and goal for production, defoliation management by animal (continuous
or rotational grazing), and surplus forage management (Shewmaker & Bohle, eds., 2010).
Because grazing animals is a dynamic soil-plant-animal system, consideration of soil and other
resource (water for irrigation) limitations are part of pasture management decisions (Shewmaker
& Bohle, eds., 2010). Matching animal numbers with adequate forage availability to maximize
growth, aided by pasture management, optimizes grazing systems (Schnabel et al., 2000;
Shewmaker & Bohle, eds., 2010; Lorenz, 2018; Moore et al., 2019). Several pasture
management methods improve forage production: adding legumes, irrigation, fertilization,

32

adoption of higher-production C4 grass species, and inclusion of manure (Lorenz, 2018).
Fertilization with phosphorus can increase production without spurring soil carbon
decomposition, while adding nitrogen increases plant growth and reduces legumes (Schnabel et
al, 2000). Irrigation can increase forage production but has been shown to decrease SOC stocks
and soil nitrogen levels (Mudge et al., 2016). However, the effectiveness of all these techniques
is dependent on the forage species, plant density, stage of growth, level of defoliation, and
nutrient balance before fertilization (Moore et al., 2019). Highly defoliated species in the
reproductive stage with high levels of existing nutrients will not show marked increases in
growth.
Grazing management systems range from continuous grazing that offers unfettered access
to the whole pasture to rotational grazing that moves livestock through (typically) smaller
subdivisions called paddocks. Revegetation occurs with animals onsite and often shows
excessive wear and evidence of preferential grazing in continuously grazed systems. Rotationally
grazed systems offer longer regrowth periods during the animal-excluded period (Holochek,
1983; Shewmaker & Bohle, eds., 2010). Large mammal herbivory in short duration, high density
rotation indicates higher levels of SOC than continuously grazed systems or ungrazed systems
(Khalil et al., 2019; Naidu et al., 2022; Kim et al., 2023). Adaptive multi-paddock (AMP)
grazing shows higher biomass production, improved water infiltration, and higher soil nitrogen
levels in comparison to heavy continuous grazing (Hillenbrand et al., 2019; Kim et al., 2023),
conditions that support higher soil carbon levels. Studies of light continuous or rotational grazing
show contradictory results. Preferential grazing in light grazing systems leads to overgrazing in
some areas and increases undesirable and invasive species in other areas, impacting plant growth
and carbon inputs. (Hillenbrand et al., 2019). Loss of animal treading to initiate decomposition in

33

light grazing systems decreases SOC levels (Schnabel et al., 2000). On the other hand, light
grazing causes the least reduction in SOC in some systems, particularly where there is adequate
water and high-growth C4 grasses (Bai and Cotrufo, 2022).
Treading and trampling by animal hooves impacts soil structure directly by changing the
form and stability of soil aggregates, which affects bulk density, pore size and soil strength. The
degree of influence depends on animal class, soil type, vegetation response to climate conditions,
and pasture management system. Dry animal-trampled soil results in aggregate crushing and
smaller aggregates at the surface of the soil. Moist soils are compressed by animal hooves,
resulting in the collapse of larger soil pores and higher bulk density. On saturated soils, animal
hooves can poach the soil, creating compacted lumps of soil. Both dry and moist trampling
effects alter water infiltration, which potentially increases flooding and runoff and decreases
water holding and plant productivity (Taboada et al., 2011). While infiltration rate may be a
sensitive indication of soil physical health, soil texture (sandy soils are resistant to compaction)
and organic matter content—above and belowground—may reduce the impacts of grazing on
soil physical properties (Taboada et al., 2011). In continuously grazed systems, physical soil
changes are influenced by moisture content, soil type, and stocking rates, although even in
lightly stocked pastures, uneven trampling near water sources or other high-traffic areas can
impact erosion and compaction rates (Taboada et al., 2011). Rotational grazing impacts on soil
physical properties are mixed, depending on soil type and climatic factors such as water for
vegetative regrowth (Taboada et al., 2011). Harm to soil physical properties may be mediated by
natural precipitation cycles, plant growth and senescence, and exclusion of grazers, although
some rotational systems may not allow a full natural recovery cycle (Taboada et al., 2011). In
silty loam and clay loam soils with high organic matter (>4.5%), infiltration rate and soil

34

macropores showed the greatest improvement (up to 127%) after grazing stopped and visible
signs of poaching decreased 50% within 87 to 165 days. Additionally, macroinvertebrates
(earthworms) create macropores naturally in the top 5cm of soil in areas of manure deposition.
(Taboada et al., 2011). Recovery in drier climates may take several years for full recovery, which
is not a problem in temperate climates like Southwest Washington.
2.5 Conclusion
Soil organic carbon levels are determined by the interplay between soil physical,
chemical, and biological properties. Soil is a dynamic ecosystem that is highly responsive to
management practices. Systems that increase carbon inputs without impeding soil functions,
such as rotational grazing and management of soil pH may show higher carbon soil levels than
those who retard plant growth after grazing and decrease diversity.
Recent interest in soil carbon sequestration highlights the need to identify systems and
practices that optimize carbon storage for long time periods. Variations in climate and edaphic
factors make development of best management practices (BMPs), indicators, and assessment
extremely challenging. Long term studies in Wisconsin showed that rotationally grazed systems,
in contrast to cropping systems, sequestered carbon at 0-15cm depth. All systems in the study
lost SOC across the whole 0-90cm soil profile, however, calling into question realistic climate
mitigation potential of soil carbon sequestration (Sanford et al., 2012). A repeat of the study in
2022 looked at SOC at 0-30cm depth and found 18% - 29% higher SOC MAOM in pastures than
cropping systems, findings that were validated by comparison to studies in similar systems
globally. This is due to the continuous, undisturbed input of high-quality inputs from animals and
root matter that promotes carbon accrual and impedes decomposition or SOC loss. (Rui et al.,
2022). Although Rui et al. (2022) did not test at the same depth as Sanford et al. (2012),

35

recognition that most soil biological responses occur in the top 30 cm of soil may have driven the
focus on the upper profile. A third study found that cool-season perennial pasture with
undisturbed soils offers the best soil carbon sequestration mitigation potential (Becker et al.,
2022). In each of these studies, the soil types and climate are very different from Southwest
Washington, making direct inference unlikely. The conditions identified for high soil carbon
sequestrations, however, indicate that Southwest Washington pasturelands may offer significant
climate mitigation potential.
Furthermore, soil organic carbon depends on the relationship between climate, inherent
soil properties, and management—disturbances and inputs (Schjonning et al., 2004). The
variability of highly heterogeneous soil conditions requires careful collection and monitoring of
valid soil data and management practices across time. Adoption of land-based carbon
sequestration receives only 2.5% of mitigation funding (Griscom et al., 2017) Uncertainty about
potential and cost, the longevity of stored carbon, and social and cultural barriers hinder
widespread acceptance of soil carbon sequestration. Consistency in measurement, baseline
information, and calibration of equipment, tests, and methods ensures valid data about soil
carbon levels (Olson, 2013). This study provides baseline data, consistent with WSDA State of
the Soils assessment, on soil organic carbon levels and management practices in Southwest
Washington pasturelands.

36

Chapter 3. Methods
In this two-part study, I used management practice surveys and in situ soil sampling to
compare the effects of management practices on soil organic carbon (SOC) levels and bulk
density (BD) in Southwest Washington pasturelands. The management practice surveys
completed by landowners in January and February 2023 provided historical information to
contextualize the soil data. I collected in-field soil samples to measure soil organic matter, soil
pH, cation exchange capacity, nutrients, and bulk density (BD) in pastures and native prairies
during a two-week period in late winter. Statistical analysis compared the effects of management
on SOC levels and BD in different soil series.
Participants in the study were recruited from the Southwest Washington Grazing
Association and from Thurston County community members who had submitted pasture soils for
testing to Thurston Conservation District in 2020 and 2021. Both populations were emailed a
request to participate in the study. Those who agreed were not compensated for their
participation; however, a complimentary copy of the soil test and the results of the study were
provided to each participant. The survey, approved by the Evergreen State College Institutional
Review Board, was released on 3 January 2023 to participants. The survey obtained information
regarding practices such as grazing, haying, fertilization, irrigation, weed control, soil
amendment applications, length of practices, and history of land use. The specific questions
included in the survey can be found in Appendix 1. All responses were captured by 10 February
2023. I clarified some responses with follow up email or phone calls. All responses (with
identifying information redacted) are included in Appendix 2.
In-field sampling followed the Washington State Department of Agriculture Standard
Operating Procedures (WSDA, 2022). Samples were collected to assess baseline soil organic

37

carbon levels and bulk density. Eight soil cores were extracted from each of 5 random points
within a pasture, then homogenized prior to testing, yielding one sample for each pasture. I used
Google Earth Pro to establish a polygon outlining the pasture, dropped five pins to establish the
five sampling points, and downloaded the maps onto my handheld device (Google, n.d.). The
maps allowed me to navigate the pasture to the collection points. To collect the samples, I
removed any plant material (grass, roots, and crop residue) on the top 1-2 inches, then inserted a
7/8-inch diameter soil-sampling probe at a 90-degree angle to the surface of the soil to a depth of
12 inches (30.48 cm), a depth compatible with the IPCC (Abdalla et al, 2018). I deposited the
soil from the probe into a clean, non-galvanized bucket. I homogenized the pasture sample by
mixing the soil in the bucket with gloved hands. I placed at least two cups of soil into a labeled
and resealable plastic bag and stored it in a refrigerator at 40oF before shipment to Midwest
Laboratories within seven days from collection (Figure 1).
Soil organic matter, nutrients, pH, and cation exchange capacity (S1A) tests and Total
Carbon tests were done by Midwest Laboratories, 13611 B Street, Omaha, NE, 68144. Midwest
Laboratories are fully accredited in Washington state through the NAPT program (Midwest,
n.d.). Time constraints and laboratory proficiency were the determining factors in selection of
Midwest to conduct these tests. Soil organic matter (SOM) was determined by loss on ignition
and is expressed as a percentage. Total carbon (TC) was measured from dry combustion on a
LECO analyzer, indicates both SOC and soil inorganic carbon (SIC), and is also expressed as a
percentage. Although all soil data was shared with participants, nutrients were not examined in
this study. Complete soil test results are listed in Appendix 3.

38

Figure 1.
Bulk density corer and step probe with 12” (30.08 cm) soil core

As a soil physical indicator relevant to root establishment, plant growth, and soil carbon
stocks, I collected bulk density cores at three random points co-located with the five sampling
points within the pasture. To collect soil for BD testing, I used a 6” soil core cup containing three
2” rings, attached to a compact slide hammer, to obtain samples from a 4-inch (0.10 m) depth
(Figure 1). A single core from the center ring was carefully pared from the outer two rings and
placed into a lidded disposable aluminum baking cup after all visible rocks or large organic
matter was removed. The three BD specimens collected from each site were refrigerated at 40oF
until analyzed. I measured bulk density in the Evergreen State College Science Support Center
laboratory by drying the cores in their aluminum cups for 24 hours at 105oC in a Yamato
DKN602C oven. I weighed each dried sample in its cup and then weighed each empty cup with a
Sartorius Group Acculab analytical balance to determine the net mass of the dried sample

39

(Figure 2). The known volume of each core was 102.96296 cm3 (2" x 2" cylinder). The data for
bulk density is expressed in g soil/cm3.

Figure 2.
Bulk density samples in the lab

Note. From left to right: Bulk density sample on the analytical balance; Bulk density samples in
the oven; Bulk density samples in the desiccator waiting to be weighed.
For each site, organic matter percentage was multiplied by both the van Bemmelen Index
(0.58) and a more conservative constant (0.50) to determine soil organic carbon content
(Franzluebber, 2010; Pribyl, 2010; Heaton et al., 2016; Weil and Brady, 2017).
Statistical analysis was done using R version 4.1.3 (2022-03-10)— “One Push-Up”
Copyright © 2022) The R Foundation for Statistical Computing Platform: x86_64-w64mingw32/x64 (64-bit). Correlation, linear regression, and ANOVA examined the relationship
between edaphic factors, land management, and livestock management on SOC levels. The alpha
level α = 0.10 was used to determine significance. Significant findings were further analyzed
with Tukey’s HSD to determine which factors are most influential. Shapiro tests to determine

40

normality of the data were used. Those data that were not normally distributed were log10
transformed.

41

Chapter 4. Results
Despite a relatively small geographical area—just over 182,000 hectares (450,000
acres)—and a small sample size (26 sites), extraordinary diversity in site characteristics, land
use, livestock, and management practices complicated analysis. The sites were distributed across
five counties—Pierce, Thurston, Mason, Grays Harbor, and Lewis—and consisted of 23 pastures
and three restored native prairies (Figure 3). Pasture and prairie sample areas ranged from 0.3
acres to 17.5 acres, with a median of 3.3 acres and mean of 5.3 acres. Three prairie sites and one
pasture site were selected as controls. Although there is a long history of dairy farming in the
control pasture, rare prairie species have been documented onsite, and organic and floral speciesprotective practices have been followed for many years.

42

Figure 3.
Google map of survey sites

Note. The range of sites includes Pierce, Thurston, Mason, Grays Harbor, and Lewis counties.
4.1 Habitat type outcomes
Heterogeneity in soil series both within and among the 26 sites included 36 different soil
series as either single, dominant series or components of complexes. Sample areas were selected

43

within the pastures and prairies to be representative of single dominant soil series rather than
multiple soil series. Soil series descriptions from USDA/NRCS Web Soil Survey were combined
with data from Google Earth Pro and EDIT (Ecosystem Dynamics Interpretive Tool) Ecological
Site Descriptions (ESD). The sites in this study include soils from the Puget Lowlands Forest
(Ecological Site AX002X01X004), Puget Lowlands Moist Forest (Ecological Site
AX002X01X005), Puget Lowlands Prairie (Ecological Site AX002X01X006), Puget Lowlands
Wet Forest (AX002X01X007), and Puget Lowlands Riparian Forest (Ecological Site
AX002X01X008) groups (Table 2). Although these descriptions are not complete within the
EDIT system, soils grouped within have similar ecological characteristics, including similar
ranges of MAP and MAT, similar parent materials, and similar vegetation. Parent materials
included glacial outwash, glacial drift, volcanic ash, shale, sandstone, siltstone, alluvium, loess,
lacustrine and glaciomarine deposits, igneous rocks, and herbaceous organic deposits. Soil
textures within each ESD were more consistent, while variations between ESD were markedly
different.
Table 2.
Site Characteristics
Site ID

County

ESD

Prairie1

Thurston

PLP

Prairie 2

Pierce

PLP

153

14.8

Prairie 3

Thurston

PLF

232

3.5

Prairie 4

Lewis

PLMF

60

17.5

Thurston PLWF

2

1.3

S2TEST23

Acres Acres
total sampled
580
15.9

44

Dominant Soil
Series
Spanaway (60%)Nisqually (30%)
Complex
Spanaway gravelly
sandy loam (100%)
Everett (50%)Spanaway (35%)
Complex
Doty silt loam
(90%)
Skipopa silt loam
(90%)

Subordinate Soil
Series
--

-Nisqually (10%),
Semiahoo (5%)
Klabe (5%),
Lacamas (5%)
Yelm (10%)

TCSCAS23

Thurston

PLF

10

5.4

LCCHEH23

Lewis

PLRF

7

2.2

TCGENI23

Thurston PLWF

24

5.3

TCMEDI23

Thurston

PLRF

5

1.8

TCCLMA23

Thurston PLWF

3

2.4

TCPRAI23

Thurston

PLP

50

5.9

TCROCH23

Thurston

PLP

1

0.3

TCTENI23

Thurston

PLP

2.5

1.2

LCLINC23

Lewis

PLMF

7

4.6

GHBLAC23

Grays
Harbor

PLRF

8

2

TCBLAC23

Thurston

PLF

23

2.2

TCYELM23

Thurston

PLF

10

8.1

TCWOOD23 Thurston

PLF

1

1

20

12.1

TCSCAW23

Thurston PLMF

MCJONE23

Mason

PLWF

5

2.6

TCCENT23

Thurston

PLP

12

3.4

45

Everett very
gravelly sandy
loam (80%)
Chehalis silty clay
(90%)
Everson clay loam
(85%)

Puyallup silt loam
(85%)

Alderwood (10%),
Indianola (10%)
Alvor (5%), Reed
(5%)
Everson (5%),
McKenna (5%),
Cagey (3%),
Bellingham (2%)
Newberg (5%),
Semiahoo (3%),
Sulta (2%)
Yelm (10%)

Skipopa silt loam
(90%)
Spanaway gravelly
-sandy loam (100%)
Spanaway gravelly
-sandy loam (100%)
Spanaway gravelly
-sandy loam (100%)
Melbourne loam
Scamman (5%)
(95%)
Elma (65%)Scatter (10%),
Fordprairie (20%)
Roundtree (5%)
complex
Everett very
Alderwood (10%),
gravelly sandy
Indianola (10%)
loam (80%)
Kapowsin stony
Norma (2%)
loam (85%)
Alderwood gravelly Indianola (5%),
sandy loam (85%) Everett (5%),
Shalcar (3%),
Norma (2%)
Yelm fine sandy
Everson (5%),
loam (85%)
Norma(5%),
Skipopa (3%)
Bellingham silt
-loam (100%)
Spanaway gravelly
-sandy loam (100%)

TCSCAN23

Thurston

PLP

1

0.7

TCCHEH23

Thurston

PLP

8

2.1

GHCHEH23

Grays
Harbor

PLRF

17

15

TCMIMA23

Thurston

PLP

4

3.2

TCTEHS23

Thurston PLMF

79

4.4

Nisqually fine
loamy sand (85%)
Spanaway gravelly
sandy loam (100%)
Maytown (45%)Chehalis (30%)Rennie (15%)
complex
Spanaway (60%)Nisqually (30%)
complex
Cathcart gravelly
loam (100%)

Yelm (3%),
Norma (2%)
-Scatter (5%),
Elma (5%)

--

--

Note. ESD= Ecological Site Description: PLP = Puget Lowlands Prairie, PLF = Puget Lowlands
Forest, PLMF = Puget Lowlands Moist Forest, PLWF = Puget Lowlands Wet Forest, PLRF =
Puget Lowlands Riparian Forest.
Climatically, mean average temperatures (MAT) are warmest in Thurston County
(10.7oC), followed by Grays Harbor (10.1oC), Mason (9.6oC), and Lewis (8.8oC) Counties.
Pierce County MAT is coolest at 8.4oC (NOAA NCEI, 2023). Mean average precipitation
(MAP) ranges from 1,300 mm in Thurston County to 1,674 mm in Pierce, followed by 1,867 mm
in Lewis, 2,344 mm in Mason, and 2,461 mm in Grays Harbor Counties (NOAA NCEI, 2023).
Surprisingly, given the high MAP for this region and the February sampling window, the soil at
most sites was at field capacity. Only two sites had saturated soil during the sampling period:
TCGENI23 and MCJONE23.
The most influential factor in every analysis of SOC in this study derived from the ESD
(p < 0.01) (Figure 4). This is closely related to the findings of soil texture influence on SOC
explained in Section 4.3. Climatic influences on SOC levels in each ESD was not included in this
study but may in the future provide more information about potential for SOC sequestration in
each ESD.

46

Figure 4.

Soil Organic Carbon % (log10)

ESD Effect on Soil Organic Carbon

Ecological Site Description
Note. From left to right on the x-axis: Puget Lowlands Forest, Puget Lowlands Moist Forest,
Puget Lowlands Prairie, Puget Lowlands Riparian Forest, Puget Lowlands Wet Forest.
4.2 Bulk density outcomes
In the realm of physical soil properties, SOC is moderately negatively correlated with the
average bulk density of each site. (Pearson’s r= -0.4632, F1,24 = 6.212, p = 0.02) (Figure 5). This
finding is consistent with other studies indicating higher bulk density values are associated with
lower SOC levels.

47

Figure 5.

Soil Organic Carbon % (log10)

Linear Regression of Soil Organic Carbon with Bulk Density.

Bulk Density Average (g/cm3)

The effect of ESD on BD was statistically significant (p= 0.06), although the large variation in
PLWF values was likely due to two hydric soil types that were the only saturated soils sampled
during the study. (Figure 6). Additionally, two outliers in sample the PLP data contributed to the
unusual results. On occasion, large pieces of organic matter may have been undetected in the BD
sample, such as the large chunks of wood in this (rejected) from one of the PLP sites (Figure 7).

48

Figure 6.

Bulk Density (g/cm3)

ESD Effect on Bulk Density

Ecological Site Description
Note. From left to right: Puget Lowlands Forest, Puget Lowlands Moist Forest, Puget Lowlands
Prairie, Puget Lowlands Riparian Forest, Puget Lowlands Wet Forest.
Tukey’s HSD post-hoc assessment showed the effect of PLF was significantly different from the
effect of PLWF (p = 0.10) on BD. There were no significant differences for the other ESD
effects.

49

Figure 7.
Bulk Density Core Example

Note. This sample with large chunks of organic matter was discarded.
The relationship between BD and weed management by mechanical means suggests
lower BD with weed control including mechanical means. However, two of the three BD outliers
in this data set coincide with the mechanical weed management, which may skew these results
(Figure 8).

50

Figure 8.

Bulk Density (g/cm3)

Weed Management Effect on Bulk Density

Weed Management Practices
Note. CM = Chemical and mechanical (n=4). M = Mechanical (n=12). Other practices not
included in statistical analysis CMF= Chemical, mechanical, and fire (n=2), CF= Chemical and
Fire (n=2), C=Chemical (n=1), and MF= Mechanical and Fire (n=1).

The influence of grazing styles on bulk density measurements, while not statistically significant
at p = 0.19, suggests that rotational grazing styles result in lower BD measurements than other
grazing styles (Figure 9). Another noteworthy point is the higher BD measurement for WG,
which includes pastures utilized for hay.

51

Figure 9.

Bulk Density (g/cm3)

Grazing Style Effect on Bulk Density

Grazing Style
Note. C365= Continuous 365 days annually (n= 3), CS=Continuous seasonal April-October
(n=2), R365=Rotational 365 annually (n=4), RI=Rotationally integrated (n=9), WG= Wild
grazing (n=8).
4.3 Soil physical and chemical outcomes
The relationship between SOC levels and soil silt and clay percentages were most notable
of the inherent soil property findings. There was strong negative correlation between SOC and
percentage of silt (Pearson’s r = -0.51, F1,24 = 8.27, p < 0.01) (Figure 10) and between SOC and
percentage of clay in the soil (Pearson’s r = -0.48, F1,24 = 7.362, p = 0.01) (Figure 11). These

52

findings were unexpected. Soils with higher sand content typically do not have high levels of
organic carbon because sand particles do not support large accumulations of organic matter.

Figure 10.

Soil Organic Carbon % (log10)

Linear regression of Soil Organic Carbon with Soil Silt Percentage

Soil Silt Percentage

53

Figure 11.

Soil Organic Carbon % (log10)

Linear regression of Soil Organic Carbon with Soil Clay Percentage

Soil Clay Percentage (log10)
The chemical soil properties tested in this study, pH and cation exchange capacity (CEC),
did not have a strong association with SOC (p = 0.84 and p = 0.11, respectively). Cation
exchange capacity (CEC) (log10) had a moderate negative correlation (Pearson’s r = -0.32) to
SOC (Figure 12). This is an unusual finding, because typically CEC has a positive correlation
with SOC due to its positive correlation to clay. In this study, clay and silt are negatively
associated with higher SOC, corroborating the negative CEC association with SOC.

54

Figure 12.
Linear Regression of Soil Organic Carbon with Cation Exchange Capacity

Soil Organic Carbon % (log10)

Pearson’s r = -0.32
p = 0.11

Cation Exchange Capacity (meq/100g) (log10)
4.4 Land management outcomes
Survey information obtained from land managers addressed length of practices and
history of land use (Table 3), as well as irrigation, fertilization, weed management, and soil
amendments (Table 4). Participants reported a wide variety of land uses prior to 2003, including
forestry, prairie, hay, row cropping, and pasture for horses, beef and dairy cattle, and sheep.
Current management practices began in a wide range of periods, from more than 50 years ago to
this year. Participants at six sites started their current practices 20 or more years ago, eight began
between 10 and 19 years ago, and twelve participants began their current practices less than 10
years ago. Some of the sites have complicated histories, however, such as the native restored

55

prairies, which were farmed, utilized for various military training purposes, and overrun with
invasive species in the past 170 years. Current practices for the prairie sites reflect records for
invasive species removal and treatment, as well as restoration efforts. Among the permutations
of pre-2003 history of the pastures in this study, ANOVA revealed a strong effect from pre-2003
history as a native prairie versus as a hay field or pasture (p = 0.02) on SOC levels (Figure 13).
Limited replicates of reported pre-2003 history as a site for row cropping (2 sites), mow and
fallow (1), or permutations of historical use precluded statistical analysis of those options.

Figure 13.

Soil Organic Carbon % (log10)

Prior History Effect on Soil Organic Carbon

History Prior to 2003
Note. H= hay (n=3), N= native prairie (n=3), P= pasture (n=12). Other history not included in
statistical analysis PH= pasture and hay (n=2), FP= forest and pasture (n=1), F= forest (n=1),
PH= pasture and hay (n=2), FPH= forest, pasture, and hay (n=1), MFa= mow and fallow (n=1),
HRC= hay and row crops (n=1), RC= row crops (n=1), NA= no answer (n=2).

Analysis of the effects of the start of current practices were confounded by two
participants who began in 2023 (resulting in a duration of 0 years, which causes errors in R).
56

Adding 0.1 to each value reported for the start of current management practices yielded a
moderate positive correlation (Pearson’s r = 0.33) between the SOC level and the start of current
practices, at a statistically significant level (p= 0.10) (Figure 14).

Figure 14.
Linear Regression of Soil Organic Carbon with Start of Current Practices

Soil Organic Carbon % (log10)

Pearson’s r = 0.33
p= 0.10

Start of Current Practices (log10)

57

Table 3.
Site Use
Site ID

ESD

Pre-2003 History

Start of Current
Practices

Primary Use

Prairie1

PLP

N

15.1

U

Prairie 2

PLP

N

13.1

U

Prairie 3

PLF

N

11.1

U

Prairie 4

PLMF

P

20.1

G

S2TEST23

PLWF

FPH*

11.1

G

TCSCAS23

PLF

P

1.1

G

LCCHEH23

PLRF

P

7.1

G

TCGENI23

PLWF

PH *

2.1

G

TCMEDI23

PLRF

HRC*

1.1

G

TCCLMA23

PLWF

P

20.1

G

TCPRAI23

PLP

P

2.1

G

TCROCH23

PLP

FA*

1.1

G

TCTENI23

PLP

H

20.1

G

LCLINC23

PLMF

P

1.1

U

GHBLAC23

PLRF

FP*

13.1

G

TCBLAC23

PLF

H

17.1

H

TCYELM23

PLF

F*

0.1

G

TCWOOD23

PLF

P

3.1

G

TCSCAW23

PLMF

P

20.1

G

MCJONE23

PLWF

PH*

5.1

H

TCCENT23

PLP

RC*

6.1

G

TCSCAN23

PLP

P

3.1

G

TCCHEH23

PLP

P

G

GHCHEH23

PLRF

P

13.1
17.1

TCMIMA23

PLP

FAM*

20.1

U

TCTEHS23

PLMF

P

56.1

G

58

H

Note. Pre-2003 History: N= native prairie, P= pasture, FPH= forest, pasture, and hay, H= hay,
PH= pasture and hay, HRC= hay and row crops, FA= fallow, RC= row crops, FAM= fallow and
mow. Start of current practices is number of years from completion of the management survey
in 2023 plus the addition of 0.1. Primary use: U= unmanaged pasture or native restored prairie,
G= grazing, H= hay. Items marked with * were not included in statistical analysis.
Participants at 22 of 26 sites practice weed management of some sort, a mix of grazing
and cover cropping (biological), mechanical (hand pulling, mowing, digging, brush cutting, weed
whacking, hoeing), chemical (herbicides), and fire (prescribed burning). The limited number of
replicates for biological and several combinations of other practices precluded statistical
analysis. However, for the sites that used mechanical alone and chemical and mechanical
together, those management practices affected SOC levels to a statistically significant degree (p
= 0.05) (Figure 15). Most participants employed a combination of weed management practices.
Figure 15.

Soil Organic Carbon % (log10)

Weed Management Effect on Soil Organic Carbon

Weed Management Technique
Note. CM= Chemical and mechanical control (n=4), M= Mechanical control (n=12). Other
practices not included in statistical analysis: C= chemical (n=1), CF= chemical and fire (n=2),
MF= mechanical and fire (n=1), and CMF= chemical, mechanical, and fire (n=2).
Questions about dominant forage species yielded disparate answers. Commonly known
species are orchardgrass (Dactylis glomerata), tall fescue (Schedonorus arundinaceus), white
59

clover (Trifolium repens), ryegrass (Lolium perenne and Lolium multiflorum), bent grass
(Agrostis capillaris), Kentucky bluegrass (Poa pratensis), reed canary grass (Phalaris
arundinacea), meadow foxtail (Alopecurus pratensis), tall oatgrass (Arrhenatherum elatius), and
subterranean clover (Trifolium subterraneum). Six participants reported unknown species, while
others named pasture grass/mix, prairie species, mixed Pacific Northwest grass, and native grass.
Without a specific foliage assay of each site to confirm, this data was unable to be analyzed
effectively in this study.
In a similar vein, for the nine seeded pastures and two seeded prairies, records of seed
species varied widely. Among the seeds applied to pastures are bird’s foot trefoil (Lotus
corniculatus), blue wildrye (Elymus glaucus), chicory (spp), tetramag rye, t-raptor (turnipxrape),
redtop turnip, peas, oats, clover (Trifolium repens and Trifolium subterraneum), fescue
(Schenodorus aundinaceus), orchardgrass (Dactylis glomerata), Kentucky bluegrass (Poa
pratensis), and a 42-species cover crop mix. Seeding data on the two native restored prairies
included a single species on one site and more than 67 species on the other. Without comparable
data from each site, effective analysis is limited. Indeed, ANOVA indicated no relationship
between seeding and SOC levels. Future studies examining the species richness of each site and
its impact on SOC may offer insight into another significant factor in SOC levels in SW WA
pasturelands, as some evidence indicates that the sites with the highest species richness may also
be the sites with highest SOC levels.
In the five grazing and three hay renovated pastures—an uncommon practice—only two
grazing pastures were tilled, whereas all three hay pastures were tilled. One grazing participant
indicated no renovation but reported tilling and seeding with an unknown seed species in the
past. On the other hand, one of the most common land management practices is fertilization, with

60

12 of 23 pastures fertilized according to soil tests (5 pastures), with annual application of dairy
manure (three pastures), via chicken tractor (1 pasture), or using a commercial fertilizer (1
pasture). Only six of 23 pastures are irrigated, and only four of 23 employ soil amendments such
as lime (3), gypsum (2), imported liquid dairy (3) or chicken (1) manure, compost (1), and potash
(1). As a note: restored native prairies are not managed for fertility, irrigated, or treated with soil
amendments and were not assessed for these practices. ANOVA tests revealed no notable effects
on SOC levels from fertilization, irrigation, soil amendments, pasture renovation, or tilling (p =
0.68) (Figure 16).
Figure 16.

Soil Organic Carbon % (log10)

Land Management Effect on Soil Organic Carbon

Land Management Practices
Note. F= Fertilization (n=3), PTIFA= Pasture renovation, tilling, irrigation, fertilization, and soil
amendments (n=3). Other land management practice combinations not included in statistical
analysis include: P= Pasture renovation (n=1), PIF= Pasture renovation, irrigation, and
fertilization (n=1), FA= Fertilization and soil amendments (n= 1), PTF= Pasture renovation,
tilling, and fertilization (n=2), TIF= Tilling, irrigation, and fertilization (n=1), PF= Pasture
renovation and fertilization (n=1), and I= Irrigation (n=1).
61

4.5 Land use outcomes
Primary uses of the pasturelands included five unmanaged and/or native restored prairie
(U) sites, three hay (H) sites, and 18 grazing (G) locations (Figure 17.) Grazing styles are
comprised of wild grazing (WG) [physical signs of deer and/or elk at prairies, unmanaged, and
hayed sites provided evidence that, in keeping with Lorenz (2018), almost all undeveloped lands
are grazed by wildlife], continuous seasonal (CS) grazing [typically during the growing season
from April-October], continuous all year without rest (C365), rotationally grazed all year (R365),
and rotationally integrated (RI) grazing [pastures are grazed in rotation during the growing
season, with pasture rest periods appropriate for forage growth and defoliation, livestock species,
weather conditions for forage growth and recovery, and other restrictions, such as deferment for
ESA species] (Figure 18).

62

Figure 17.

Soil Organic Carbon % (log10)

Current Primary Use Effect on Soil Organic Carbon

Note. Unmanaged includes native restored prairies and sites not utilized for grazing or hay.

63

Figure 18.

Soil Organic Carbon % (log10)

Grazing Style Effect on Soil Organic Carbon

Grazing Style
Note. C365= Continuous grazing 365 days annually (n= 3), CS= Continuous seasonal grazing
April to October (n=2), R365= Rotational grazing 365 days annually (n=4), RI= Rotationally
integrated grazing based on animal forage needs, forage growth and defoliation rates, and other
considerations (n= 9), WG= Wild grazing by deer and elk (n=8).
Primary use as an unmanaged or native restored prairie (U) resulted in substantially
higher levels of SOC than either grazing (G) or hay (H) (p=0.05). However, the combination of
hay (where wild grazing occurs) with unmanaged pastures and native restored prairies created a
64

much larger variation in SOC levels when sites are assessed by grazing style. While the results
were not statistically significant for grazing style influences on SOC (p = 0.18), all rotationally
based grazing styles (including hay, where large amounts of biomass are removed from the
ecosystem) showed markedly more SOC than either of the continuously grazed systems.
Evidence of herbivores such as feces and elk and deer tracks suggested at least some
grazing was likely at every site, including those whose primary purpose was listed as hay,
leading to the animal designation of deer and elk at sites that do not have human managed
livestock. Farm animals in this study included dairy and beef cattle, sheep, goats, chickens,
geese, guinea fowl, and ducks. Although livestock variations complicated statistical analysis,
they are representative of pastoral operations in SW WA (USDA NASS, 2017). To compare
species while there is some evidence that species impact on SOC differs, in this study I used
Animal Unit Equivalents (AUE) to convert different species into a comparable reference frame
(Pate et al., 2022). With an adjusted range of values from 0.1 to 100 AUE on the 18 sites with
reported livestock, analysis indicated a slight negative influence of higher AUE on SOC levels,
although not to a statistically significant level (Pearson’s r = -0.38, F1,16= 2.724, p = 0.12)
(Figure 19). Future studies with more consistent animal species deployed on the sites and less
variation of AUE within the species may offer more insight into the effects of animal density on
SOC.

65

Figure 19.

Soil Organic Carbon % (log10)

Linear Regression of Soil Organic Carbon with Animal Unit Equivalent

Pearson’s r = -0.38
p = 0.12

Animal Unit Equivalent (log10)

Different animal species exerted different influences on the SOC levels in the soil. Higher
levels of SOC were associated with deer and elk and sheep, in comparison with cattle, although
none to a statistically significant degree (p = 0.12) (Figure 20). The effect of animal species on
SOC levels was largest for deer and elk versus cattle (p = 0.10) and sheep versus cattle (p =
0.37).

66

Figure 20.

Soil Organic Carbon % (log10)

Animal Species Effect on Soil Organic Carbon

Animal Species
Note. C= Cattle (n= 9), D= Deer and elk (n= 8), S= Sheep (n= 4). Other species not included in
statistical analysis: Goats (n=2), Horses (n= 1), Chickens (n=1), Guinea fowl/Ducks/Geese (n=
1).

67

Table 4.
Land Management Practices
Site ID

ESD

LM

WM

Grazing
Style

Prairie1

PLP

--

CMF*

Prairie 2

PLP

--

CF*

Prairie 3

PLF

--

CF*

Prairie 4

PLMF

--

C*

S2TEST23

PLWF

--

M

TCSCAS23

PLF

--

CM

LCCHEH23

PLRF

PTIFA

M

TCGENI23

PLWF

--

M

TCMEDI23

PLRF

--

--

TCCLMA23

PLWF

F

CM

TCPRAI23

PLP

PIF*

M

TCROCH23

PLP

--

M

TCTENI23

PLP

FA*

--

LCLINC23

PLMF

--

--

GHBLAC23

PLRF

--

CM

TCBLAC23

PLF

PTIFA

M

TCYELM23

PLF

PTF*

--

TCWOOD23

PLF

TIF*

C

TCSCAW23

PLMF

PTF*

M

MCJONE23

PLWF

PF*

M

TCCENT23

PLP

F

M

TCSCAN23

PLP

I*

M

TCCHEH23

PLP

F

MF*

GHCHEH23

PLRF

PTIFA

CMF*

WG

D

NA

TCMIMA23

PLP

--

CM

WG

D

NA

TCTEHS23

PLMF

--

M

CS

C

NA

WG
WG
WG
RI
R365
C365
RI
RI
RI
RI
RI
C365
RI
WG
RI
WG
R365
C365
CS
WG
R365
RI

68

R365

Animal
Species
D
D
D
C
CSCh*
C
C
C
CS*
C
C
Ch*
S
D
C
D
S
Ho*
C
D
SGDuGeGu*
S
SG*

AUE

NA
NA
NA
30.0
0.6
100.0
60.0
45.5
36.0
9.0
90.0
0.1
2.1
NA
24.0
NA
4.0
2.5
7.0
NA
11.0
1.4
5.0

Note. LM (Land management practices): PTIFA= pasture renovation, tilling, irrigation,
fertilization, and soil amendments, F= fertilization, PIF= pasture renovation, irrigation, and
fertilization, FA= fertilization and soil amendments, PTF= pasture renovation, tilling, and
fertilization, TIF= tilling, irrigation, and fertilization, PF= pasture renovation and fertilization, I=
irrigation. WM (Weed management practices): CMF= chemical, mechanical, and fire, CF=
chemical and fire, C= chemical, M= mechanical, CM= chemical and mechanical. Animal
species: D= deer/elk, C= cattle, S= sheep, Ch= chickens, Du= ducks, G= goats, Ge= geese, Gu=
guinea fowl, Ho= horses. AUE (Animal Unit Equivalent): one cow-calf pair= 1.0, one yearling
cattle= 0.60, one mature sheep= 0.20, one broiler chicken= 0.008 (Pate, et al., 2022). Items
marked with an * were not included in statistical analysis.
4.6 Confounding factors
Confounding factors include the selection bias of the participants, who were either
members of the Southwest Washington Grazing Association or previous soil testing clients of
Thurston Conservation District. Both groups have a demonstrated level of commitment to
agricultural education and implementation of new techniques or processes. At the very least, they
demonstrated enough interest in their soil to submit samples for analysis and asked for
recommendations to improve their soil health. Another source of bias is the large proportion of
cattle ranchers who participated in the study. The effects of bovine (11 participants) versus ovine
(7), caprine (2), equine (1), or poultry (3) herbivory may impact study results. Also unmeasured
is the effect of deer and elk, signs of which were observed, but the number of animals was not.
Bulk density measurements correlated as expected with SOC, despite complications arising in
the sampling. One site with hydric soil yielded BD measurements more than 50% lower than the
expected NRCS Web Soil Survey values, likely due to saturated soil. In other cases, rocks within
the BD core were not visible until after the sample was dried and weighed. Similarly, BD cores
with occluded organic matter such as large wood chunks or root masses also may have produced
skewed results. The final confounding factor in field sampling arose from the gravelly soils at
several sites. Full insertion of the 12-inch step probe was not always possible, although every
reasonable effort was made to consistently sample to the full 12-inch depth.
69

Chapter 5. Discussion
5.1 Soil organic carbon
Ecological site description (ESD) was the most influential factor for SOC. Soils within
the Puget Lowlands Prairie (PLP) contained significantly higher SOC levels than Puget
Lowlands Forests (PLF), Puget Lowlands Moist Forests (PLMF), Puget Lowlands Wet Forests
(PLWF), or Puget Lowlands Riparian Forests (PLRF). Given the sandy, gravelly nature of most
soils within the PLP ESD, this was a surprising result. High sand content is typically associated
with low soil organic matter (and thus SOC levels), while silt and clay are associated with high
SOC content (Li et al., 2017; Weil and Brady, 2017). This unexpected outcome is corroborated
by strong negative correlations with clay to SOC levels for all ESDs.
While the negative correlation between bulk density and SOC in this study replicated
other findings (Van Haveren, 1983; Reganold, 1988; Sakin, 2012; Li et al., 2017; Weil and
Brady, 2017), the results were again unanticipated. Typically, high sand content soils have
higher bulk density and thus lower SOC; in this case, the higher sand content soils did not follow
expectations with SOC results. It is a somewhat surprising finding, because the overwhelmingly
high SOC ESD was PLP, which has low silt and clay percentages and high sand content. BD is
usually higher in sandy soils because there is less organic matter and less pore space in sandy
soils than in finely textured soils (Weil and Brady, 2017). In this case, however, PLP bulk
densities were on par with PLRF and substantially lower than PLF and PLMF, although two
outlier low values (of nine total PLP sites) may have skewed the data. The exceptionally low
values of the PLWF were likely due to soil saturation at one site and undetected large chunks of
organic matter in the sample at another. Regardless, bulk density results in this study were

70

consistent with expectations, in that bulk density values negatively correlated with SOC levels.
The twist is that the soil textural profiles did not support bulk density or SOC expectations.
There are a few possible explanations for the unusual findings. The SOC levels for nearly
all sites were high, ranging from 2.1% at the lowest end to 12.7% at the highest. In contrast,
expected NRCS Web Soil Survey values vary from 1.4% to 5.3%. Climatic conditions (high
mean annual precipitation and temperate mean annual temperatures) favor continual vegetative
growth for most of the year while limiting microbial decomposition and loss of SOC (Stockman
et al., 2012). Overall, SOC levels in Southwest Washington in this study offer a promising
premise for SOC sequestration.
One explanation for the higher levels of SOC in PLP soil may rest with the allocation of
vegetative carbon in prairies versus forests. Up to 50% of the carbon produced from
photosynthesis in a prairie or grassland is stored belowground in the root structure (Dass et al.,
2018), whereas the carbon in a forest system is largely in the aboveground biomass (Bachelet et
al., 2011; Case et al., 2021). When the forest is converted into pasturelands, the forest SOC
pool—not as large as a prairie to begin with—is depleted (Khalil et al., 2019). The pre-2003
history of the sites corroborates SOC accumulation, with high SOC levels for sites identified as
previous native prairies or pastures, and mostly low SOC levels in prior forest or hay fields. The
positive correlation between the start of the current practices—the length of time as a pasture—
and SOC supports the hypothesis that SOC recovery after forest conversion is possible, although
perhaps a long-term process.
Another possible explanation for high SOC levels in low clay and silt percentage soils
such as the PLP soils is that the SOC in the PLP soils is particulate organic matter (POM) rather
than mineral associated organic matter (MAOM). Limited clay and silt in PLP soils provide less

71

surface area or chemical bond potential for organic matter (organic carbon) to accumulate as
MAOM in the soil. Although POM is considered less stable than MAOM, it is not constrained by
saturation limits of clay and silt particles and can continue to accrue in the soil (Conant et al.,
2017 as quoted in Mayerfeld, 2023; Bai and Cortrufo, 2022). Land management practices and
climatic factors such as temperature and precipitation are POM vulnerabilities, which will be
discussed later in this document. Nevertheless, this finding is a positive indicator for SOC
sequestration potential in the PLP soils. The findings in this study do not provide SOC
sequestration rates; however, replication of this study over time will determine the SOC
sequestration rate.
A third possible explanation is the species diversity in PLP versus the forested ESDs.
Particularly in mature forests, understory species are limited by competition for resources and
photosynthetic occlusion (Pojar and MacKinnon, 1994; Brockway, 1998) while open grasslands
historically can host over 250 species (Dunwiddie et al., 2007). Unfortunately, this study did not
have the appropriate data to make this assessment.
A final possible explanation for the unexpectedly high SOC levels in PLP is the history
of anthropogenic burning of native prairies (Pojar and MacKinnon, 1994; Dunwiddie and
Bakker, 2011). Four of the five sites that reported weed management by fire have PLP soils;
although it was not included in the analysis, another PLP site also reported fire within the last
twenty years. Although regular burning has not been widely practiced in the past 150 years or
more, charcoal from fires persists for thousands of years. Ancient Indigenous burning practices
on the native prairies likely left an enduring legacy of high soil carbon levels on the historical
native prairies.

72

5.2 Land management
Analysis of land management practices—weed management, pasture renovation,
renovation tilling, fertilization, irrigation, seeding, and application of soil amendments—revealed
that their effects on SOC were less distinct than those of ESD. Fertilization, irrigation, seeding,
and application of soil amendments did not influence SOC levels in this study. These practices
were not uniformly utilized across the sites, and thus were not an effective predictor of SOC
levels in this study.
Control of weeds by mechanical means was more influential on SOC levels than control
by chemical and mechanical means. Although the inclusion of fire as a weed management
technique was not statistically analyzed because the number of replicates was too low, the sites
incorporating fire as a weed management tool were high in SOC. This result is likely because
three of five sites indicating fire as a weed management tool were PLP sites; two of those three
had the highest SOC scores. The third highest SOC level, also a PLP site, has a history of fire,
although it was not a datapoint for analysis. In addition to legacy SOC from historical
anthropogenic burning, current practices seem to enhance SOC in gravelly, sandy soils that
would not be expected to have high SOC levels.
Pasture renovation showed little influence on SOC, unless renovation tilling was
employed. Of the six sites that practiced renovation tilling, all were forested ESD. While I did
not analyze the direct relationship between forested ESD sites that tilled to renovate the pasture
and SOC, there seemed to be a negative impact of this practice on SOC.
5.3 Land use
All the sites in this study are currently grassland. Historical use, prior to 2003, influenced
SOC levels significantly for prior native prairies and pastures, although history as a forest or as a

73

hayfield was not influential. This is likely due to the loss of SOC due to land use conversion
from forest to pasture and the removal of biomass carbon from the hayfield without return of
carbon to the soil through animal deposition or plant senescence. Current land use, including
unmanaged pastures, grazing pastures, and hayfields, showed a strong relationship between
primary use and SOC levels. Unmanaged pastures, including the native restored prairies, were
significantly higher in SOC than either grazing or haying sites. Four of the five unmanaged sites
were PLP with high SOC levels, suggesting that two use factors influenced SOC: ESD and
leaving as much biomass as possible in the pasture instead of removing the organic materials
through haying or animal consumption.
Time elapsed since beginning the current practices showed a moderate positive
correlation with SOC levels. Although the findings were not statistically significant (p = 0.103),
use of the land as a pasture, for grazing, hay, or unmanaged and/or native prairies, had a positive
effect on SOC as the organic matter slowly builds in a pasture and accumulates over time. While
unmanaged and/or native restored prairies had the highest levels of SOC, the continuous, diverse
vegetative coverage, integration of livestock (or wild ungulates), and lack of soil disturbance in
pasturelands over time should expand SOC for all Southwest Washington soils in this study.
Grazing style influence on SOC in pastures was complicated by the combination of hay
sites with unmanaged sites wherein both are “wild grazed” by deer and elk. The decision to
include “wild grazing” as a category rather than exclude hay sites as ungrazed acknowledges the
impact of grazing on all pastures, although the added removal of biomass carbon from the hay
sites reduces organic material and thus SOC on those sites. Although not statistically significant,
rotational grazing styles were associated with higher SOC levels than continuous grazing styles.
Rotational 365 (R365) and wild grazing (WG) were associated with a higher but not statistically

74

significant SOC level than continuous seasonal (CS) grazing, and SOC levels at PLP sites were
statistically significant compared to all four forested ESDs. The results suggest grazing styles
with adequate forage recovery periods, particularly on PLP soils, allow SOC to accumulate to a
higher level than continually grazing for the length of the growing season.
In addition to the grazing style, there is some indication the number of animals grazed
exerts an influence on the SOC level. This study used the term AUE (animal unit equivalent) to
express the relative quantity of animals grazing. Although not statistically significant, the
number of animals grazing bears further examination to determine the appropriate stocking rate
to maximize SOC levels. A more dominant factor in this study is the species of animal grazing.
The negative relationship between cattle and SOC may be in part explained by ESD, because
only one PLP site grazes cattle, whereas three of four sites each in PLMF, PLRF, and PLWF
graze cattle. In contrast, four PLP sites graze sheep, three have deer/elk, and one grazes chickens,
in general representing low AUE values. Lower SOC levels in forest soils as a group influence
the low cattle/SOC association. If one does not have the benefit of PLP pastures, grazing at lower
AUE, in a rotational or WG-like manner, appears most likely to result in higher SOC.

75

Chapter 6. Conclusion
Historically, fire was used to manage prairies for open space, floral species cultivation,
and other purposes. The PLP pastures in this study had significantly higher SOC than the other
pasture ESDs. While there may be other factors contributing to the higher SOC levels in the PLP
pasture, the legacy of Indigenous burning is likely a primary influence. Proximity to human
assets and infrastructure, unprecedented fuel loads, and suburban and rural sprawl can preclude
large-scale burning at many sites to maintain or enhance SOC levels. An effective alternative is
to utilize carefully managed rotational grazing to support diverse species and curtail invasives
(Khalil et al., 2019). However, one must avoid practices such as renovation tilling and
overstocking animals. Furthermore, while grazing in moist cool climates may decrease SOC in
some areas (Abdallah et al., 2018), properly managed rotational grazing in Southwest
Washington pasturelands has great potential to preserve and sequester soil organic carbon.

76

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Appendix 1 Management Survey Questions
1. Email address
2. Informed Consent
You are being invited to participate in a research survey titled “Management Survey for
Southwest Washington Pasturelands.” This study is being conducted as part of a Master of
Environmental Studies thesis project at Evergreen State College. The purpose of this survey is
to understand how management practices impact soil organic carbon levels and bulk density in
pasturelands. If you agree to take part in this study, you will be asked to complete a
management survey. This survey will ask questions regarding your pasture management
practices. It will take you approximately thirty minutes to complete.
There will be no compensation for participating in this survey. As a token of our gratitude,
land managers will receive an individual soil health report in mid-2023. We expect that your
participation in the study will help increase understanding of the impacts of pasture
management practices on soil health parameters in the Southwest Washington area.
Risks to you are minimal and are likely to be no more than mild discomfort with sharing your
pasture management practices. To the best of our ability, your answers in this study will
remain confidential. Your participation in this study is completely voluntary and you can
withdraw at any time. You are free to skip any question that you choose. Data collected from
you for this project will be combined across all respondents. Results will not be reported in a
way that makes individuals identifiable. Any personally identifying information will be
removed before your information is shared.

If you have questions about this project or if you have a research-related problem, you may
contact the researcher, Christina Wagner, MES candidate, Evergreen State College at 111-2223333 or christina.wagner@evergreen.edu. If you have any questions concerning your rights as
a research subject, or you experience problems as a result of participating in this research
project, you may contact the Evergreen State College Human Subjects Research Committee
with any concerns that you have about your rights or welfare as a study participant. This office
can be reached by email at irb@evergreen.edu.

By clicking “I agree” below you are indicating that you are at least 18 years old, have
read and understood this consent form and agree to participate in this research study.
Please print a copy of this page for your records.
3. Farm name
4. Name of Land Manager
5. Farm Address
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6. Date Survey Completed
7. Unique Sample Identification for Pasture (assigned by researcher)
8. Please describe the land use prior to its current use. (Example: Continuous dairy field 19501995. Fallow 1995-2004. Rotational beef cattle 2005-present)
9. When did the pasture begin to be used in its CURRENT capacity? (Unsure, or select prior to
2003, 2003-2022)
10. How many TOTAL acres do you manage, including fallow and other pastures or fields?
11. How many acres are in THIS pasture?
12. What percentage of your operation is managed similarly to this specific pasture? (Select 110%, 11-20%...91-100%)
13. What year did you begin managing this specific pasture?
14. What is the PRIMARY use of this pasture? (Hay, Grazing, Unmanaged)
15. Please estimate which years you hayed this pasture (Please select all that apply)(Unsure,
prior to 2003, 2003-2022)
16. For grazed pastures, what is the dominant forage species?
17. For grazed pastures, have you seeded this pasture?
18. For SEEDED pastures, what have you seeded and when?
19. What animal species graze this pasture? (Please select all that apply) (Cattle, sheep, horses,
hogs, chickens, other)
20. If you answered Other to “What animal species grazes this pasture?” please explain here.
21. How many times a year do you typically graze this pasture? Please indicate number of passes
per month or season.
22. What is the typical length of grazing period in this pasture? Please be as specific as possible.
If all parts of the pasture are managed the same way, this question asks how you manage a single
paddock or partition. It does not require the sum of all grazing cells. (Example: 3 consecutive
days two times a month Apr-June, 1 day two times a month Jul, no grazing Aug-Sep, 3 days two
times a month Oct, no grazing Nov-Mar)
23. What is the length of the rest period in this pasture? Please be as specific as possible.
(Example: 24 rest days a month Apr-Jun, 30 rest days Jul, rest Aug-Sep, 24 days a month Oct, no
grazing Nov-Mar)
24. For the grazing cells described above, how many animals were grazed during the periods
identified? Please be as specific as possible.
25. Please estimate what years this pasture was grazed in this manner. (Select all that apply)
(Prior to 2003, 2003-2022)
26. If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. (Example: 30% replanted in 2010 due to rust.) (If this pasture has not
been replanted please enter N/A)
27. When you renovated this pasture, did you till?
28. Please estimate what years you tilled this pasture to renovate. (Please select all that
apply)(Prior to 2003, 2003-2022)
29. When you renovated this pasture, how many tillage passes did you make on average?
30. What is your primary tillage implement? (Example: Chisel plow, field cultivator, Offset disk)

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31. Please estimate what years you sub-soiled (deep ripped) this pasture. (Select all that
apply)(Never, Prior to 2003, 2003-2022)
32. Is this pasture certified?
33. What certifications apply to this pasture? (Please check all that apply)(Organic, Salmon Safe,
Farmed Smart, Other)
34. Please indicate the year(s) you received each certification(s).
35. If you answered Other to “What certifications apply to this pasture?” please explain here.
36. Do you manage weeds in this pasture?
37. How do you manage weeds in this pasture? (Please select all that apply)(Chemical control,
Mechanical control, Green mulch, Cover crops, Other)
38. Please describe weed control. (Example: Brush cut followed by glyphosate application once
in spring annually)
39. Do you irrigate this pasture?
40. How do you determine water needs in this pasture? (Select all that apply)(Calculating
evapotranspiration, Evaluating by site, Evaluating by infrared, Same rate nearly every year, Soil
moisture by feel method, Soil moisture sensors, Other)
41. If you answered Other to “How do you determine water needs in this pasture?” please
explain here.
42. Please estimate the number of acre-inches (ac-in) applied to this pasture in a typical year.
43. Do you fertilize this pasture?
44. How do you decide what rate to fertilize this pasture? (Please select all that apply)(Plant
tissue samples, Same rate for entire farm based on annual soil tests, Different rates for different
part of farm based on soil tests, Precision nutrient application, Same rate every year, Other)
45. If you answered Other to “How do you determine what rate to fertilize this pasture?” please
explain here.
46. Do you ever add any soil amendments to this pasture (NOT including crop residues, cover
crops, or manure from livestock integration)?
47. Have you added lime to this pasture?
48. Please estimate what years you applied lime and average rate applied. (Example: 2 tons/acre
in 2010 and 2017)
49. Have you added gypsum to this pasture?
50. Please estimate what years you applied gypsum and average rate applied. (Example: 2
tons/acre in 2010 and 2017)
51. Have you added manure (trucked in) to this pasture?
52. Please estimate what years you applied manure, source of manure (unsure, chicken, dairy
cow, feedlot cattle, hog, sheep, slurry, other), and average rate applied. (Example: 50lbs/acre
chicken manure in 2010 and 2017)
53. Have you added compost to this pasture?
54. Please estimate year compost was applied, average rate applied, and if known, carbon to
nitrogen ratio (unsure, 10, 15, 20, 25, other). (Example, 2009—50lbs/acre applied 2009, C:N 10,
2015—100lbs/acre, C:N unsure)
55. Have you added biochar to this pasture?

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56. Please estimate year(s) biochar was applied and average rate applied (Example: 50lbs/acre
applied 2009)
57. Have you added biosolids to this pasture?
58. Please estimate year(s) biosolids were applied and average rate applied. (Example: 50lbs/acre
applied 2009)
59. Have you added humic acids to this pasture?
60. Please estimate year(s) humic acids were applied and average rate applied. (Example:
50lbs/acre applied 2009)
61. Have you added microbial inoculants to this pasture?
62. Please estimate year(s) microbial inoculants were applied and average rate applied.
(Example: 50lbs/acre)
63. Have you added other soil amendments to this pasture?
64. Other soil amendments. Please describe type, year(s) of application and rate of application.
65. Did you (or do you) receive any cost share or incentive funding to add soil amendments?
66. Is there anything else you would like us to know about his pasture (Optional)

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Appendix 2 Management Survey Responses
PRAIRIE 1
Has this prairie ever been seeded? Yes
Which species? 67 seeded species
When? 2008, 2009, 2010, 2011, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021,
2022
Do you manage weeds on this prairie? Yes
Please describe weed control. Burned 2012, 2014, 2016. Sprayed triclopyr (not for last 5 years),
spot treat tall oat grass with clethodium. Manually pull scotch broom.
PRAIRIE 2
Has this prairie ever been seeded? No
Which species?
When?
Do you manage weeds on this prairie? Yes
Please describe weed control. Glyphosate for tall oat grass 2015, 2018; sulphur cinquefoil
Milestone 2009, 2010, Garlon 3A 2007, 2011, 2012, 2015, 2018, Vastlan 2020, 2022. Spot spray
all. Burned 2009, 2011, 2014, 2016, 2019
PRAIRIE 3
Has this prairie ever been seeded? Yes. Did not overlap with sample area.
Which species? Fescue
When? 2013
Do you manage weeds on this prairie? yes
Please describe weed control. Burned 2011, 2012, 2016
PRAIRIE 4
Please describe land use prior to its current use. Dairy pasture
What year did the land begin to be used in its CURRENT capacity? Prior to 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? 100+
How many acres are in THIS pasture? 60
What percentage of your operation is managed similarly to this specific pasture? 81-90%
What year did you begin managing this specific pasture? 1967
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Prairie species
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Cattle
How many times in a year do you typically graze this pasture? Varies due to weather and
deferment periods
What is the typical length of grazing period in this pasture? Varies due to weather and species
protection
What is the length of the rest period in this pasture? April thru August

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For grazing cells described above, how many animals were grazed during the periods identified?
30
Please estimate what years this pasture was grazed in this manner. Prior to 2003- 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. No
When you renovated this pasture, did you till? No
Is this pasture certified? Yes
What certifications apply to this pasture? Organic
Please indicate the year(s) you received each certification. 2001-present
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Please describe weed control. Cutting, weed hoe, manual pulling
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
S2TEST23
Please describe land use prior to its current use. Overgrazed with cows, sheep and goats on a
fixed pasture rotation or potentially continuous. Likely haying at times. 100 years ago this was a
logging headquarter site, potentially log yard.
What year did the land begin to be used in its CURRENT capacity? Prior to 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 2
What percentage of your operation is managed similarly to this specific pasture? 71-80%
What year did you begin managing this specific pasture? 2012
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Tall fescue
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Cattle, sheep, chickens
What is the typical length of grazing period in this pasture? Rotationally grazed
For grazing cells described above, how many animals were grazed during the periods identified?
3
Please estimate what years this pasture was grazed in this manner. 2012, 2013, 2014, 2015, 2016,
2017, 2018, 2019, 2020, 2021, 2022
Please estimate what years you till this pasture to renovate. Prior to 2003
Please estimate what years you sub-soiled (deep ripped) this pasture. Never
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control, Other
Please describe weed control. Seasonally timed grazing by a mixture of animal types. Cows first
when available. Sheep clean up. Wintertime sheep grazing of blackberry followed by manual
weed whacking of canes. High moving of Canada thistle following July grazing.
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No

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TCSCAS23
Please describe land use prior to its current use. Continuous grazing with beef cattle 1940’s-2020
What year did the land begin to be used in its CURRENT capacity? 2022
How many TOTAL acres do you manage, including fallow and other pastures or fields? 100+
How many acres are in THIS pasture? Pastures are 5-50 acres each. Follow up email: 5
What percentage of your operation is managed similarly to this specific pasture? 71-80%
What year did you begin managing this specific pasture? 2020
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Orchard grass, rye grass
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Cattle
How many times a year do you typically graze this pasture? Pastures are grazed 5-10 times,
some are continuously grazed. Follow up email: continuous
What is the typical length of grazing period in this pasture? April-October
What is the length of the rest period in this pasture? 15-30 days
For grazing cells described above, how many animals were grazed during the periods identified?
I have several different herds on several different pastures, it depends. Follow up email: 100
Please estimate what years this pasture was grazed in this manner. 2018, 2019, 2020, 2021, 2022
WSU overseeded some native seed on the study pasture around 2019?
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Chemical control, Mechanical control
Please describe weed control. Spot chemical spray, hand pull, mowing
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
LCCHEH23
Please describe land use prior to its current use. Continuous dairy pasture ground
What year did the land begin to be used in its CURRENT capacity? 2020
How many TOTAL acres do you manage, including fallow and other pastures or fields? 100+
How many acres are in THIS pasture? 7
What percentage of your operation is managed similarly to this specific pasture? 31-40%
What year did you begin managing this specific pasture? 2016
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? No dominant species
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? 2020 tetramag rye, stf 43, forb
feast chicory, t-raptor, red top turnip, approximately 3 lbs/acre of each
What animal species graze this pasture? Cattle
How many times in a year do you typically graze this pasture? 7-9 on a 21 day rotation
What is the typical length of grazing period in this pasture? Strip graze according to season and
stubble height. May cut if needed.
What is the length of the rest period in this pasture? 21-24 April through October. No grazing
Nov-April
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For grazing cells described above, how many animals were grazed during the periods identified?
60
Please estimate what years this pasture was grazed in this manner. 2016, 2017, 2018, 2019, 2020,
2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. 2020 was total new renovation including plowing
When you renovated this pasture, did you till? Yes
Please estimate what years you tilled this pasture to renovate. 2020
When you renovated this pasture, how many tillage passes did you make on average? 1 plow, 2
disk, 1 drill, 1 cultipac
What is your primary tillage implement? Moldboard plow 2 bottom
Please estimate what years you sub-soiled (deep ripped) this pasture. Never
Is this pasture certified? Yes
What certifications apply to this pasture? Organic
Please indicate the year(s) you received this certification. 1998 til present
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control, Cover crops
Please describe weed control. Manual hoe as needed
Do you irrigate this pasture? Yes
How do you determine water needs in this pasture? Evaluating by site. Evaluating by infrared.
Same rate nearly every year. Soil moisture by feel method
Please estimate the number of acre-inches applied to this pasture in a typical year. 6
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Same rate for entire farm based on annual
soil tests
Do you ever add any soil amendments to this pasture? Yes
Have you added lime to this pasture? No
Have you added gypsum to this pasture? Yes
Please estimate what years and average rate applied. 2022 150lbs/acre
Have you added manure (trucked in) to this pasture? Yes
Please estimate what years you applied manure, source of manure, and average rate applied.
Dairy will let you know
Have you added compost to this pasture? No
Have you added biochar to this pasture? No
Have you added biosolids to this pasture? No
Have you added microbial inoculants to this pasture? No
Have you added other soil amendments to this pasture? No
Did you (or do you) receive any cost share or incentive funding to add soil amendments? No
TCGENI23
Please describe land use prior to its current use. ~1950-2021 continuous grazing and hay, 2021present rotational grazing
What year did the land begin to be used in its CURRENT capacity? 2021
How many TOTAL acres do you manage, including fallow and other pastures or fields? 30-100
How many acres are in THIS pasture? 24
What percentage of your operation is managed similarly to this specific pasture? 91-100%
102

What year did you begin managing this specific pasture? 2021
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Cool season mix (Tall fescue, meadow
foxtail, orchard grass, reed canary grass)
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Cattle
How many times in a year do you typically graze this pasture? Once
What is the typical length of grazing period in this pasture? We put cows in 0.5 acre paddocks
for 3-5 days from July-Oct
What is the length of the rest period in this pasture? 360-362 days
For grazing cells described above, how many animals were grazed during the periods identified?
45 in 2021, 36 in 2022
Please estimate what years this pasture was grazed in this manner. 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A (as far as I know)
When you renovated this pasture, did you till? No
Please estimate what years you till this pasture to renovate. 2020
Is this pasture certified? Yes
What certifications apply to this pasture? Organic
Please indicate the year(s) you received this certification. 2022
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Please describe weed control. Hand pull tansy, blackberry, and thistle throughout the year with a
heavy focus on spring and early summer
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
TCMEDI23
Please describe land use prior to its current use. Hay and row crops
What year did the land begin to be used in its CURRENT capacity? 2021
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 5
What percentage of your operation is managed similarly to this specific pasture? 41-50%
What year did you begin managing this specific pasture? 2022
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Pasture grasses
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Cattle, sheep
How many times in a year do you typically graze this pasture? 5 passes a year
What is the typical length of grazing period in this pasture? Grazed Mar-Sep 4 days in each
paddock
What is the length of the rest period in this pasture? 25 days in summer, then from Oct til March
For grazing cells described above, how many animals were grazed during the periods identified?
30 sheep, 1 cow
Please estimate what years this pasture was grazed in this manner. 2022
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When you renovated this pasture, did you till? No
Is this pasture certified? No
What certifications apply to this pasture? Organic
Please indicate the year(s) you received this certification. 1998 til present
Do you manage weeds in this pasture? No
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
TCCLMA23
Please describe land use prior to its current use. Pasture
What year did the land begin to be used in its CURRENT capacity? Prior to 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? ~3
What percentage of your operation is managed similarly to this specific pasture? 91-100%
What year did you begin managing this specific pasture? 1994
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Cool season grasses, fescue, orchard
and rye
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Cattle
How many times in a year do you typically graze this pasture? 1-2 times a month
What is the typical length of grazing period in this pasture? Pasture is sectioned into cells with
one strand electrical fence. In the spring when the grass is growing fast, the pasture is strip
grazed. Fences are moved twice a day. In the summer when growth is slowed down the animals
are grazed in larger cells. Depending on the number of animals land the grass, the animals are
moved when the grass length dictates it.
What is the length of the rest period in this pasture? The rest period is dependent on the rate of
growth of the grass. Again, it is an approximately 3-acre pasture divided up into cells with a
strand of electrical poly wires. In the spring the rate of movement is faster with strip grazing so
the rest period can be up to 35 days. Again, this id dependent on the number of head of cattle that
are grazing at that time. In the summer there is a sacrifice area that is over grazed while the slow
growing grass in the other parts of the pasture recover.
For grazing cells described above, how many animals were grazed during the periods identified?
It can range from 5 to a mix of cow/calves and yearlings up to 13 head
Please estimate what years this pasture was grazed in this manner. 2003-2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. No
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Please describe weed control. Manual pull undesirable plants. Will spot spray for Canadian
thistle this spring
Do you irrigate this pasture? No
Do you fertilize this pasture? Yes
104

How do you decide what rate to fertilize this pasture? Spread aged manure to needed areas. Also
use 16-16-16 commercial fertilizer
Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? The cattle are
taken off the pasture in the winter. Given the clay soil it is the best way to preserve the health of
the grasses.
TCPRAI23
Please describe land use prior to its current use. Year round cow calf operation
What year did the land begin to be used in its CURRENT capacity? 2021
How many TOTAL acres do you manage, including fallow and other pastures or fields? 100+
How many acres are in THIS pasture? 50
What percentage of your operation is managed similarly to this specific pasture? 51-60%
What year did you begin managing this specific pasture? 2021
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Rye and not sure
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? Inter seeded rye
What animal species graze this pasture? Cattle
How many times in a year do you typically graze this pasture? Total of 12 passes over a 7 month
period with the early spring the fastest rotation
What is the typical length of grazing period in this pasture? Start approximately April first and
end late September early October
What is the length of the rest period in this pasture? April through June 15 to 20 days after July
30 days-I strip graze and move the fence every day sometimes 2 a day based on grass and
consumption, so it varies and it is a learned trait not a set system
For grazing cells described above, how many animals were grazed during the periods identified?
150 calves 400-800 lbs
Please estimate what years this pasture was grazed in this manner. 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. I inter seeded all pastures with rye and clover mix
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Do you irrigate this pasture? Yes
How do you determine water needs in this pasture? Evaluating by site. Check soil for depth of
water penetration
Please estimate the number of acre-inches applied to this pasture in a typical year. No idea
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Same same for entire farm based on annual
soil tests
Do you ever add any soil amendments to this pasture? No
TCROCH23
Please describe land use prior to its current use. No management
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What year did the land begin to be used in its CURRENT capacity? 2022
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 1
What percentage of your operation is managed similarly to this specific pasture? 71-80%
What year did you begin managing this specific pasture? 2022
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Unknown
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Chickens
How many times in a year do you typically graze this pasture? Free range (30 passes per month)
What is the typical length of grazing period in this pasture? All year
What is the length of the rest period in this pasture? No rest
For grazing cells described above, how many animals were grazed during the periods identified?
4-7 chickens
Please estimate what years this pasture was grazed in this manner. 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Please describe weed control. Hand pull tansy and scotch broom
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
Did you (or do you) receive any cost share or incentive funding to add soil amendments? No
Is there anything else you would like us to know about the history of this pasture? Unmanaged
pasture, not previously grazed, small chicken flock grazing since December 2022
TCTENI23
Please describe land use prior to its current use. Hay production
What year did the land begin to be used in its CURRENT capacity? 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 2.5
What percentage of your operation is managed similarly to this specific pasture? 91-100%
What year did you begin managing this specific pasture? About 2003, not exactly sure when we
completely fenced it
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? I don’t know
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? About 2010-bird’s foot trefoil,
chicory, and white clover. Only seeded about 1/3 of the field. 2022-a commercial sheep-oriented
pasture mix
What animal species graze this pasture? Sheep
How many times in a year do you typically graze this pasture? Pasture is cross fenced. Starting in
March or April we rotationally graze
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What is the typical length of grazing period in this pasture? March-June grazing till grass is only
4”, on to next etc. Total of 6 areas. Return to first as it seems appropriate. Each sub-pasture is
usually good for 3-6 days, depending on the herd size in a given year.
What is the length of the rest period in this pasture? Depends
For grazing cells described above, how many animals were grazed during the periods identified?
Between 7-14
Please estimate what years this pasture was grazed in this manner. 2007, 2008, 2009, 2010, 2011,
2012, 2013, 2014, 2017, 2018, 2019, 2020, 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A
When you renovated this pasture, did you till? No
Is this pasture certified? Yes
What certifications apply to this pasture? Greener World
Please indicate the year(s) you received this certification. Last 3
Do you manage weeds in this pasture? No
Do you irrigate this pasture? No
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Different rates for different part of the
farm based on soil tests
Do you ever add any soil amendments to this pasture? Yes
Have you added lime to this pasture? Yes
Please estimate what years you applied lime and average rate applied. 2017 and 2022, lime
added per suggestions from soil tests
Have you added gypsum to this pasture? No
Have you added manure (trucked in) to this pasture? No
Have you added compost to this pasture? Yes
Please estimate year compost was applied, average rate, and if known, carbon to nitrogen ratio.
Unsure
Have you added biochar to this pasture? No
Have you added biosolids to this pasture? No
Have you added microbial inoculants to this pasture? No
Have you added other soil amendments to this pasture? No
Did you (or do you) receive any cost share or incentive funding to add soil amendments? No
LCLINC23
Please describe land use prior to its current use. Pre 2020 grazed by horses, fallow 2020-2022
What year did the land begin to be used in its CURRENT capacity? 2022
How many TOTAL acres do you manage, including fallow and other pastures or fields? 30-100
How many acres are in THIS pasture? 7
What percentage of you operation is managed similarly to this specific pasture? 91-100%
What year did you begin managing this specific pasture? This parcel was purchased in
November 2022 and won’t be actively managed until spring 2023
What is the PRIMARY use of this pasture? Unmanaged
GHBLAC23

107

Please describe land use prior to its current use. One area was logged in 1998; one pasture was
hay. Approx 2007-2009 used for cattle
What year did the land begin to be used in its CURRENT capacity? 2010
How many TOTAL acres do you manage, including fallow and other pastures or fields? 30-100
How many acres are in THIS pasture? 8
What percentage of your operation is managed similarly to this specific pasture? 71-80%
What year did you begin managing this specific pasture? 2010
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Mixed fescue/bent/reed
canary/rush/sedge
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? Blue wild rye, 2013
What animal species graze this pasture? Cattle, deer and elk
How many times in a year do you typically graze this pasture? Once or twice
What is the typical length of grazing period in this pasture? 7-10 days
What is the length of the rest period in this pasture? Grazing occurs in May only
For grazing cells described above, how many animals were grazed during the periods identified?
40 yearling cattle
Please estimate what years this pasture was grazed in this manner. 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Chemical control, Mechanical control, Other
Please describe weed control. Mowing in spring and fall to deter reed canary grass; pull tansy
late spring to fall, spray Canadian thistle, bull thistle with Milestone
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
TCBLAC23
Please describe land use prior to its current use. West pastures used for continuous dairy 19671997; rotational beef cattle grazing and hay production 1998-2022. East pastures used for hay
production 1970-2022. 23 acres of west pasture converted to alfalfa/orchard grass hay production
in summer 2022
What year did the land begin to be used in its CURRENT capacity? 2022
How many TOTAL acres do you manage, including fallow and other pastures or fields? 100+
How many acres are in THIS pasture? 23
What percentage of your operation is managed similarly to this specific pasture? 21-30%
What year did you begin managing this specific pasture? 2006
What is the PRIMARY use of this pasture? Hay
Please estimate which years you hayed this pasture. 2021, 2020, 2019, 2018, 2017
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. 100% of this pasture was plowed and replated to alfalfa/orchard grass
hay production summer 2022
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When you renovated this pasture, did you till? Yes
Please estimate what years you tilled this pasture to renovate. 2022
When you renovated this pasture, how many tillage passes did you make on average? 1 pass with
bottom plow. 4 passes with disc harrow
What is your primary tillage implement? 4 bottom plow followed by offset disc harrow
Please estimate what years you sub-soiled (deep ripped) this pasture. Never
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Other
Please describe weed control. Hand pull or hoe thistle, dock, hedge mustard
Do you irrigate this pasture? Yes
How do you determine water needs in this pasture? Evaluating by site.
Please estimate the number of acre-inches applied to this pasture in a typical year. 30 ACY
annually
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Periodic application of liquid cow manure
(3 times in 5 years 2016-2021)
Do you ever add any soil amendments to this pasture? Yes
Have you added lime to this pasture? Yes
Please estimate what years you applied lime and average rate applied. .5 ton/acre in 2017, .75
ton/acre in 2021, 1.75 ton/acre in 2022
Have you added gypsum to this pasture? Yes
Please estimate what years and average rate applied. 2022 but can’t find rate applied
Have you added manure (trucked in) to this pasture? Yes
Please estimate what years you applied manure, source of manure, and average rate applied.
Liquid manure applied 2017, 2020, 2021
Have you added compost to this pasture? No
Have you added biochar to this pasture? No
Have you added biosolids to this pasture? No
Have you added microbial inoculants to this pasture? No
Have you added other soil amendments to this pasture? Yes. Potash 350lbs/acre
TCYELM23
Please describe land use prior to its current use. Forest land that was recently logged and turned
into pasture
What year did the land begin to be used in its CURRENT capacity? 2021
How many TOTAL acres do you manage, including fallow and other pastures or fields? 30-100
How many acres are in THIS pasture? 10
What percentage of your operation is managed similarly to this specific pasture? 1-10%
What year did you begin managing this specific pasture? 2021
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? PNW seed mix
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? Peas/oats cover crop planted
spring 2021, then grass mix seeded in fall 2021
What animal species graze this pasture? Sheep
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How many times in a year do you typically graze this pasture? Sheep have minimally grazed this
pasture so far
What is the typical length of grazing period in this pasture? This pasture is newly established so
grazing has been very minimal. I will let the sheep graze for a couple hours a couple of times per
week
What is the length of the rest period in this pasture? I’ll put the sheep on the pasture for a couple
hours, then rest the pasture for a few days
For grazing cells described above, how many animals were grazed during the periods identified?
20 sheep
Please estimate what years this pasture was grazed in this manner. 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. Yes 100% renovated
When you renovated this pasture, did you till? Yes
Please estimate what years you tilled this pasture to renovate. 2021
When you renovated this pasture, how many tillage passes did you make on average? 4-5
What is your primary tillage implement? 12 foot Woods disc harrow model number DHH144T
Please estimate what years you sub-soiled (deep ripped) this pasture. 2021, 2020
Is this pasture certified? No
Do you manage weeds in this pasture? No
Do you irrigate this pasture? No
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Other. We use liquid manure from a local
dairy using a 4K gallon tanker truck. We did one pass over part of the pasture so we could cover
the entire field
Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? This is a new
pasture. The prior owners logged the land and had the stumps removed and I have turned it into
new pastureland for grazing. All the topsoil was lost due to erosion so we are repairing and
rebuilding the topsoil using regenerative principles. 2023 will be the first year incorporating
livestock into the management plan.
TCWOOD23
Please describe land use prior to its current use. Native vegetation up to 1995. Pasture 1995 to
present
What year did the land begin to be used in its CURRENT capacity? Prior to 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 1
What percentage of your operation is managed similarly to this specific pasture? 91-100%
What year did you begin managing this specific pasture? 2020
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Pasture grass mix
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Horses
How many times in a year do you typically graze this pasture? Daily, 365
What is the typical length of grazing period in this pasture? 6 hours, there is no rotational grazing
control
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What is the length of the rest period in this pasture? none
For grazing cells described above, how many animals were grazed during the periods identified?
2 horses
Please estimate what years this pasture was grazed in this manner. 2020, 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A prior owner may have done something but I don’t know what
actions were taken
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Chemical control, Mechanical control
Please describe weed control. Weed cut. Glyphosate once in last 2 years
Do you irrigate this pasture? No
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Same rate for entire farm based on annual
soil tests
Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? The pastures
have always had composted manure spread periodically.
TCSCAW23
Please describe land use prior to its current use. Bought property 1969 before that was
What year did the land begin to be used in its CURRENT capacity? Prior to 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 20
What percentage of your operation is managed similarly to this specific pasture? 31-40%
What year did you begin managing this specific pasture? 1970
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Orchard grass, white clover
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? I have seeded all productive
pastures over the years but none in the last 8 to 10 years
What animal species graze this pasture? Cattle
How many times in a year do you typically graze this pasture? Summer cows are on the irrigated
pasture. After hay season they graze the hay field for about 3 months
What is the typical length of grazing period in this pasture? Cows graze the irrigated pasture all
the time from 5/1 to 8/31. Hay field graze 9/1 to mid Nov
What is the length of the rest period in this pasture? No rest
For grazing cells described above, how many animals were grazed during the periods identified?
7 cows and calves
Please estimate what years this pasture was grazed in this manner. 2008, 2009, 2010, 2011, 2012,
2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A
When you renovated this pasture, did you till? Yes
Please estimate what years you tilled this pasture to renovate. 2004
111

When you renovated this pasture, how many tillage passes did you make on average? Unknown
but lots of them
What is your primary tillage implement? Plow and disk harrow
Please estimate what years you sub-soiled (deep ripped) this pasture. 2007
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Chemical control
Please describe weed control. Spot spray to spray complete field if needed
Do you irrigate this pasture? Yes
How do you determine water needs in this pasture? Evaluating by site. Evaluating by infrared.
Same rate nearly every year.
Please estimate the number of acre-inches applied to this pasture in a typical year. N/A
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Same rate for entire farm based on annual
soil tests
Do you ever add any soil amendments to this pasture? No
MCJONE23
Please describe land use prior to its current use. 1 annual hay cutting with intermittent cattle
grazing 1942-2017
What year did the land begin to be used in its CURRENT capacity? 2018
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 5
What percentage of your operation is managed similarly to this specific pasture? 31-40%
What year did you begin managing this specific pasture? 2018
What is the PRIMARY use of this pasture? Hay
Please estimate which years you hayed this pasture. Prior to 2003-2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. About 1 acre replanted annually 1960-2017. No replantings or tillage
since then
When you renovated this pasture, did you till? Yes
Please estimate what years you tilled this pasture to renovate. Prior to 2003-2017
When you renovated this pasture, how many tillage passes did you make on average? Unsure,
was before my time here
What is your primary tillage implement? Unsure, was before my time here
Please estimate what years you sub-soiled (deep ripped) this pasture. Never
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Please describe weed control. We remove blackberry and tansy by hand (only one or two plants
of each per year)
Do you irrigate this pasture? No
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Other. We fertilize by running pastured
broilers over this field; 1 pass annually. We soil test every few years to ensure additional
applications are beneficial
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Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? Synthetic
fertilizer was historically applied from approx. 1970-2005
TCCENT23
Please describe land use prior to its current use. Farming land for past 30 years
What year did the land begin to be used in its CURRENT capacity? 2017
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 12
What percentage of your operation is managed similarly to this specific pasture? 81-90%
What year did you begin managing this specific pasture? 2017
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Pasture mix and prairie grass-native
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? High diversity mix-42 seeds
mix from Green Cover Crop-Iowa
What animal species graze this pasture? Sheep, chickens, goats, geese, guinea fowl, ducks
How many times in a year do you typically graze this pasture? 12
What is the typical length of grazing period in this pasture? 1 week
What is the length of the rest period in this pasture? 3 weeks
For grazing cells described above, how many animals were grazed during the periods identified?
55
Please estimate what years this pasture was grazed in this manner. 2018, 2019, 2020, 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. 70% replanted with special forage seeds to increase biomass production
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control, Green mulch, Cover crops
Please describe weed control. Scotch broom and blackberries either cut or uprooted
Do you irrigate this pasture? No
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Same rate every year. Just winging it and
adding lots of compost
Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? 4 orchards are
nearby and we let the animals graze in our orchards also
TCSCAN23
Please describe land use prior to its current use. Small ruminant pasture area
What year did the land begin to be used in its CURRENT capacity? 2020
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 1
What percentage of your operation is managed similarly to this specific pasture? 1-10%
What year did you begin managing this specific pasture? 2020
What is the PRIMARY use of this pasture? Grazing
113

For grazed pastures, what is the dominant forage species? Fescue, clover, orchard, rye (one other
I can’t remember)
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? We had the area cleared to
create a pasture in May 2020 with a 5 seed mix from Kiperts Feed Store
What animal species graze this pasture? Sheep
How many times in a year do you typically graze this pasture? We have 3 rotation areas for
spring through fall. We usually take them off in winter and feed them cut field hay to let the
pasture rest (Nov-March)
What is the typical length of grazing period in this pasture? We are just beginning the pasture
rotation process and learning
What is the length of the rest period in this pasture? No grazing Nov-Mar. We are working to
rest each of the three areas for 14-15 days between rotations
For grazing cells described above, how many animals were grazed during the periods identified?
We are in the middle of culling some of our flock. In Feb 2023 we will be down to 7 sheep. My
plan is to rotate them together.
Please estimate what years this pasture was grazed in this manner. 2020, 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Please describe weed control. My biggest issue is bull thistle that I have pulled by hand over the
past 3 years in the spring and throughout the summer if I find it
Do you irrigate this pasture? Yes
How do you determine water needs in this pasture? Evaluating by site.
Please estimate the number of acre-inches applied to this pasture in a typical year. Unknown
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? This property
sat abandoned for about 5 yrs. When we cleared the current pasture area we found lots of junk (a
1930s truck frame, satellite dish debris, tons of scotch broom and blackberries). We work not to
use chemicals on the property. We have protected the 3 Garry oaks that are in the pasture area
with fencing. But, in turn the sheep had kept down the weed/blackberry/Oregon grape
population. Just wish they liked bull thistle too!
TCCHEH23
Please describe land use prior to its current use. Goat dairy 1974-1995. Fallow 1995 to 2000.
Rented as horse pasture 2000-2002
What year did the land begin to be used in its CURRENT capacity? 2010
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? 8
What percentage of your operation is managed similarly to this specific pasture? 91-100%
What year did you begin managing this specific pasture? 2010
What is the PRIMARY use of this pasture? Grazing
114

For grazed pastures, what is the dominant forage species? Native grasses - unknown
For grazed pastures, have you seeded this pasture? No
What animal species graze this pasture? Sheep, Goats
How many times in a year do you typically graze this pasture? Continually
What is the typical length of grazing period in this pasture? Pasture rests for three weeks to two
months then regrazed
What is the length of the rest period in this pasture? 3 weeks to 2 months. Then grazed for 3
weeks
For grazing cells described above, how many animals were grazed during the periods identified?
20-30
Please estimate what years this pasture was grazed in this manner. 2010, 2011, 2012, 2013, 2014,
2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. Has not be renovated. One small area 20 x 30 feet was tilled by pigs for
my garden
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control, Other
Please describe weed control. Dig up and burn thistle. Sheep and goats eat scotch broom
Do you irrigate this pasture? No
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Sheep and goats fertilize naturally. Manure
from barn spread mechanically in 2009.
Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? No chemicals
have ever been applied.
GHCHEH23
Please describe land use prior to its current use. Conventional dairy
What year did the land begin to be used in its CURRENT capacity? 2006
How many TOTAL acres do you manage, including fallow and other pastures or fields? 100+
How many acres are in THIS pasture? 17
What percentage of your operation is managed similarly to this specific pasture? 21-30%
What year did you begin managing this specific pasture? 2006
What is the PRIMARY use of this pasture? Hay
Please estimate which years you hayed this pasture. Prior to 2003-2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A
When you renovated this pasture, did you till? Yes
Please estimate what years you tilled this pasture to renovate. Prior to 2003
When you renovated this pasture, how many tillage passes did you make on average? 4
What is your primary tillage implement? Plow & disc
Please estimate what years you sub-soiled (deep ripped) this pasture. Never
Is this pasture certified? Yes
What certifications apply to this pasture? Organic
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Please indicate the year(s) you received this certification. 2006
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control, Other
Please describe weed control. Pull tansy ragwort, flame weed Canadian thistle, pull wild carrot
Do you irrigate this pasture? Yes
How do you determine water needs in this pasture? Soil moisture sensors
Do you fertilize this pasture? Yes
How do you decide what rate to fertilize this pasture? Same rate every year
Do you ever add any soil amendments to this pasture? Yes
Have you added lime to this pasture? Yes
Please estimate what years you applied lime and average rate applied. 1 ton/acre 2016
Have you added gypsum to this pasture? No
Have you added manure (trucked in) to this pasture? Yes
Please estimate what years you applied manure, source of manure, and average rate applied.
Winter cow slurry 1400 gal/acre every year, chicken manure 1 ton/acre 2015
Have you added compost to this pasture? No
Have you added biochar to this pasture? No
Have you added biosolids to this pasture? No
Have you added microbial inoculants to this pasture? No
Have you added other soil amendments to this pasture? No
Did you (or do you) receive any cost share or incentive funding to add soil amendments? No
TCMIMA23
Please describe land use prior to its current use. Virginal prairie habitat covered with scotch
broom and Douglas fir until 1993. Most trees removed and scotch broom removed through hand
pulling and two applications of Rodeo herbicide (2004 and 2005). Pasture for horses 1998-2003.
Fallow from 2003 to present, mowed for fire prevention.
What year did the land begin to be used in its CURRENT capacity? 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? <30
How many acres are in THIS pasture? Unknown
What percentage of your operation is managed similarly to this specific pasture? 91-100%
What year did you begin managing this specific pasture? 1993
What is the PRIMARY use of this pasture? Unmanaged
Is there anything else you would like us to know about the history of this pasture? We have spot
sowed native forb species such as golden paintbrush, goldenrod, etc. Removing the scotch broom
has allowed many species such as camas, death camas, harvest brodiaea, to recover. It is also
heavily infested with non-native species such as ox eye daisy, tall oat grass, and redtop (poa
species). Scotch broom has not been allowed to grow or flower since 1997, but only the
herbicide treatment knocked it down for keeps. Now the property is routinely managed by
pulling every damned bit of broom that dares attempt to grow. The seed bank is slowly being
depleted, but still I pull an average of thirty to fifty plants a year total.
TCTEHS23
Please describe land use prior to its current use. Horse ranch pre-1920, rotational beef cattle
1920-present; upper forested area has been logged off three times since 1890s and used
continuously for silvipasture
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What year did the land begin to be used in its CURRENT capacity? Prior to 2003
How many TOTAL acres do you manage, including fallow and other pastures or fields? 30-100
How many acres are in THIS pasture? 79
What percentage of your operation is managed similarly to this specific pasture? 81-90%
What year did you begin managing this specific pasture? 2005
What is the PRIMARY use of this pasture? Grazing
For grazed pastures, what is the dominant forage species? Phalaris arundinacea, Arrhenatherum
elatius, Alopecurus pratensis, Schedonorus arundinaceus, Poa pratensis, Trifolium subterraneum
For grazed pastures, have you seeded this pasture? Yes
For SEEDED grazed pastures, what have you seeded and when? Pasture hasn’t been seeded
recently, but was seeded with exotic grasses and Trifolium subterraneum by my family in the
1920s
What animal species graze this pasture? Cattle
How many times in a year do you typically graze this pasture? N/A Follow up interview with
operator: continuous grazing because of missing infrastructure (cross fencing)
What is the typical length of grazing period in this pasture? N/A Follow up interview with
operator: Aug-Nov (deferment for camas)
What is the length of the rest period in this pasture? N/A Follow up interview with operator:
November to August
For grazing cells described above, how many animals were grazed during the periods identified?
30. Follow up interview with the operator: 30 cow/calf pairs
Please estimate what years this pasture was grazed in this manner. 2018, 2019, 2020, 2021, 2022
If any part of this pasture has been replanted or renovated since its original planting, please
describe when and why. N/A
When you renovated this pasture, did you till? No
Is this pasture certified? No
Do you manage weeds in this pasture? Yes
How do you manage weeds in this pasture? Mechanical control
Please describe weed control. Control blackberries, English hawthorn, and scotch broom by
brush cutting and pulling
Do you irrigate this pasture? No
Do you fertilize this pasture? No
Do you ever add any soil amendments to this pasture? No
Is there anything else you would like us to know about the history of this pasture? Details of the
grazing rotations might be better communicated through an interview with our operator or my
father, (name redacted), who is liable to talk your ear off about his great grandfather’s love of
Trifolium subterraneum and hatred for horses.

117

Appendix 3 Soil Test Results
Site ID

SOM
(%)

SOC
(50%)

TC
(%)

CEC
(meq/100g)

pH
5.3

BD Average
(g/cm3)
0.91188

P/K/Mg/Ca
(ppm)
11/58/45/261

Prairie1

25.4

12.7

15.59

2.6

Prairie 2

13.3

6.65

8.83

4.5

5.6

0.96154

7/95/59/542

Prairie 3

13.7
7.7
10.7

6.85
3.85
5.35

10.91
3.4
5.94

3.9
20.1
8.9

5.4
5.3
5.6

0.87889

13/70/53/440

2.65
2.85

3.47
2.76

7.1
25.4

5.6
5.6

0.97135
0.60763
0.95008

2/119/547/1808
18/174/113/1090
55/181/91/837

5.3
5.7
8.4
4.2
6.1

4.2
2.1
3.05

5.05
2.29
3.65

12.7
8
8.8

5.2
6.1
5.4

0.85840
0.45385

8/159/679/2676
11/82/232/1228

8.6

4.3

5.54

8.9

5.9

0.86756
0.82897
0.81898

72/31/79/1223
58/200/154/893
103/156/168/1112

TCROCH23

10.7

5.35

8.39

6.2

5.8

0.83418

23/152/93/770

TCTENI23

10.1
4.7

5.05
2.35

6.12
2.64

16.7
7.3

5.9
5.3

0.90337

29/103/330/2177

6.5

3.25

3.28

20.2

5.9

1.04232
0.87546

24/157/126/713
14/115/494/2477

9
5.2

4.5
2.6

6.36
3.63

7.2
12.9

5.2
5.7

0.85451

225/158/72/732

1.09677
1.08214

41/156/371/1345
39/162/159/846
14/50/68/639
51/47/68/450
14/413/151/747

Prairie 4
S2TEST23
TCSCAS23
LCCHEH23
TCGENI23
TCMEDI23
TCCLMA23
TCPRAI23

LCLINC23
GHBLAC23
TCBLAC23
TCYELM23
TCWOOD23
TCSCAW23
MCJONE23
TCCENT23

5
4.9
4.6

2.5
2.45
2.3

2.76
2.85
2.87

7.4
4.9
4.7

5.8
5.7
5.1

14.5

7.25

10.09

7.7

5.7

1.00444
1.02493
0.62291

TCSCAN23

10.1

5.05

5.66

4.9

5.2

0.81469

11/126/65/477

TCCHEH23

22.8

11.4

14.38

14

6.1

0.57558

59/91/163/2090

GHCHEH23

7

3.5

4.04

24.2

5.6

0.80446

9/88/515/2797

TCMIMA23

0.83832
7/76/47/479
22
11
14.3
4
5.5
TCTEHS23
5.2
2.6
2.8
9.3
5.2
0.85212
30/168/143/890
SOM= Soil organic matter, SOC= Soil organic carbon, TC= Total carbon, CEC= Cation
exchange capacity, P= Phosphorus, K= Potassium, Mg= Magnesium, Ca= Calcium

118